Mitosis and Cell Division

According to the third tenet of the cell theory, new cells originate only from other living cells. The process by which this occurs is called cell division. For a multicellular organism, such as a human or an oak tree, countless divisions of a single-celled zygote produce an organism of astonishing cellular complexity and organization. Cell division does not stop with the formation of the mature organism but continues in certain tissues throughout life. Millions of cells residing within the marrow of your bones or the lining of your intestinal tract are undergoing division at this very moment. This enormous output of cells is needed to replace cells that have aged or died. Although cell division occurs in all organisms, it takes place very differently in prokaryotes and eukaryotes. We will restrict discussion to the eukaryotic version. Two distinct types of eukaryotic cell division will be discussed in this chapter. Mitosis leads to production of cells that are genetically identical to their parent, whereas meiosis leads to production of cells with half the genetic content of the parent. Mitosis serves as the basis for producing new cells, meiosis as the basis for producing new






Kinetochores

The kinetochore is a network of protein complexes that assembles on centromere regions of chromatin and acts as the connection point between chromatids and the spindle microtubules that segregate them into daughter cells. Kinetochores must sense microtubule attachment and determine whether this attachment of sister chromatids is to the same or different spindle poles. The stable engagement of a kinetochore is regulated by the Aurora B kinase, the activity of which has been shown to reduce the affinity of several kinetochore proteins for microtubules. A single unattached or incorrectly attached kinetochore is sufficient to trigger the spindle assembly checkpoint and halt progress into anaphase, preventing cell division. 1  Once all of the chromosomes in a cell preparing to divide are correctly bioriented and satisfy the spindle assembly checkpoint, chromosomes must be pulled apart into daughter cells. An essential function of the kinetochore is to couple chromosome movement to microtubule depolymerization. Kinetochores are able to track depolymerizing microtubule ends and harness the energy released during microtubule depolymerization to move chromosomes to opposite spindle poles.

Kinetochores attach sister chromatids to the Spindle

Following the assembly of a bipolar microtubule array, the second major step in spindle formation is the attachment of the array to the sister-chromatid pairs. Spindle microtubules become attached to each chromatid at its kinetochore, a giant, multilayered protein structure that is built at the centromeric region of the chromatid (see picture below)

The kinetochore. see below (A) A fluorescence micrograph of a metaphase chromosome stained with a DNA-binding fluorescent dye and with human autoantibodies that react with specific kinetochore proteins. The two kinetochores, one associated with each sister chromatid, are stained red. (B) A drawing of a metaphase chromosome showing its two sister chromatids attached to the plus ends of kinetochore microtubules. Each kinetochore forms a plaque on the surface of the centromere. (C) Electron micrograph of an anaphase chromatid with microtubules attached to its kinetochore. While most kinetochores have a trilaminar structure, the one shown here (from a green alga) has an unusually complex structure with additional layers.




In metaphase, the plus ends of kinetochore microtubules are embedded head-on in specialized microtubuleattachment sites within the outer region of the kinetochore, furthest from the DNA. The kinetochore of an animal cell can bind 10–40 microtubules, whereas a budding yeast kinetochore can bind only one. Attachment of each microtubule depends on multiple copies of a rod-shaped protein complex called the Ndc80 complex, which is anchored in the kinetochore at one end and interacts with the sides of the microtubule at the other, thereby linking the microtubule to the kinetochore while still allowing the addition or removal of tubulin subunits at this end.



Regulation of plus-end polymerization and depolymerization at the kinetochore is critical for the control of chromosome movement on the spindle. Kinetochore attachment to the spindle occurs by a complex sequence of events. At the end of prophase in animal cells, the centrosomes of the growing spindle generally lie on opposite sides of the nuclear envelope. Thus, when the envelope breaks down, the sister-chromatid pairs are bombarded by microtubule plus ends coming from two directions. However, the kinetochores do not instantly achieve the correct ‘end-on’ microtubule attachment to both spindle poles. Instead, detailed studies with light and electron microscopy show that most initial attachments are unstable lateral attachments, in which a kinetochore attaches to the side of a passing microtubule, with assistance from kinesin motor proteins in the outer kinetochore. Soon, however, the dynamic microtubule plus ends capture the kinetochores in the correct end-on orientation



Another attachment mechanism also plays a part, particularly in the absence of centrosomes. Careful microscopic analysis suggests that short microtubules in the vicinity of the chromosomes become embedded in the plus-end-binding sites of the kinetochore. Polymerization at these plus ends then results in growth of the microtubules away from the kinetochore. The minus ends of these kinetochore microtubules are eventually cross-linked to other minus ends and focused by motor proteins at the spindle pole.





Bi-orientation is achieved by trial and error

The success of mitosis demands that sister chromatids in a pair attach to opposite poles of the mitotic spindle, so that they move to opposite ends of the cell when they separate in anaphase. How is this mode of attachment, called bi-orientation, achieved? What prevents the attachment of both kinetochores to the same spindle pole or the attachment of one kinetochore to both spindle poles? Part of the answer is that sister kinetochores are constructed in a back-to-back orientation that reduces the likelihood that both kinetochores can face the same spindle pole. Nevertheless, incorrect attachments do occur, and elegant regulatory mechanisms have evolved to correct them. Incorrect attachments are corrected by a system of trial and error that is based on a simple principle: incorrect attachments are highly unstable and do not last, whereas correct attachments become locked in place. How does the kinetochore sense a correct attachment? The answer appears to be tension .




When a sister-chromatid pair is properly bi-oriented on the spindle, the two kinetochores are pulled in opposite directions by strong poleward forces. Sister-chromatid cohesion resists these poleward forces, creating high levels of tension within the kinetochores. When chromosomes are incorrectly attached—when both sister chromatids are attached to the same spindle pole, for example—tension is low and the kinetochore sends an inhibitory signal that loosens the grip of its microtubule attachment site, allowing detachment to occur. When bi-orientation occurs, the high tension at the kinetochore shuts off the inhibitory signal, strengthening microtubule attachment. In animal cells, tension not only increases the affinity of the attachment site but also leads to the attachment of additional microtubules to the kinetochore. This results in the formation of a thick kinetochore fiber composed of multiple microtubules. The tension-sensing mechanism depends on the protein kinase Aurora-B, which is associated with the kinetochore and is thought to generate the inhibitory signal that reduces the strength of microtubule attachment in the absence of tension. It phosphorylates several components of the microtubule attachment site, including the Ndc80 complex, decreasing the site’s affinity for a microtubule plus end. When bi-orientation occurs, the resulting tension somehow reduces phosphorylation by Aurora-B, thereby increasing the affinity of the attachment site.


How tension might increase microtubule attachment to the kinetochore. ( see picture below ) These diagrams illustrate one speculative mechanism by which bi-orientation might increase microtubule attachment to the kinetochore. A single kinetochore is shown for clarity; the spindle pole is on the right. (A) When a sisterchromatid pair is unattached to the spindle or attached to just one spindle pole, there is little tension between the outer and inner kinetochores. The protein kinase Aurora-B is tethered to the inner kinetochore and phosphorylates the microtubule attachment sites, including the Ndc80 complex (blue), in the outer kinetochore as shown, thereby reducing the affinity of microtubule binding. Microtubules therefore associate and dissociate rapidly, and attachment is unstable. (B) When bi-orientation is achieved, the forces pulling the kinetochore toward the spindle pole are resisted by forces pulling the other sister kinetochore toward the opposite pole, and the resulting tension pulls the outer kinetochore away from the inner kinetochore. As a result, Aurora-B is unable to reach the outer kinetochore, and microtubule attachment sites are not phosphorylated. Microtubule binding affinity is therefore increased, resulting in the stable attachment of multiple microtubules to both kinetochores. The dephosphorylation of outer kinetochore proteins depends on a phosphatase that
is not shown here.




Following their attachment to the two spindle poles, the chromosomes are tugged back and forth, eventually assuming a position equidistant between the two poles, a position called the metaphase plate. In vertebrate cells, the chromosomes then oscillate gently at the metaphase plate, awaiting the signal for the sister chromatids to separate. The signal is produced, with a predictable lag time, after the bi-oriented attachment of the last of the chromosomes.
Multiple Forces Act on Chromosomes in the Spindle


Multiple mechanisms generate the forces that move chromosomes back and forth after they are attached to the spindle, and produce the tension that is so important for the stabilization of correct attachments. In anaphase, similar forces pull the separated chromatids to opposite ends of the spindle. Three major spindle forces are particularly critical, although their strength and importance vary at different stages of mitosis. The first major force pulls the kinetochore and its associated chromatid along the kinetochore microtubule toward the spindle pole. It is produced by proteins at the kinetochore itself. By an uncertain mechanism, depolymerization at the plus end of the microtubule generates a force that pulls the kinetochore poleward. This force pulls on chromosomes during prometaphase and metaphase but is particularly important for moving sister chromatids toward the poles after they separate in anaphase. Interestingly, this kinetochore-generated poleward force does not require ATP or motor proteins. This might seem implausible at first, but it has been shown that purified kinetochores in a test tube, with no ATP present, can remain attached to depolymerizing microtubules and thereby move. The energy that drives the movement is stored in the microtubule and is released when the microtubule depolymerizes; it ultimately comes from the hydrolysis of GTP that occurs after a tubulin subunit adds to the end of a microtubule

How does plus-end depolymerization drive the kinetochore toward the pole?  Ndc80 complexes in the kinetochore make multiple low-affinity attachments along the side of the microtubule. Because the attachments are constantly breaking and re-forming at new sites, the kinetochore remains attached to a microtubule even as the microtubule depolymerizes. In principle, this could move the kinetochore toward the spindle pole. A second poleward force is provided in some cell types by microtubule flux, whereby the microtubules themselves are pulled toward the spindle poles and dismantled at their minus ends. The mechanism underlying this poleward movement is not clear, although it might depend on forces generated by motor proteins and minus-end depolymerization at the spindle pole. In metaphase, the addition of new tubulin at the plus end of a microtubule compensates for the loss of tubulin at the minus end, so that microtubule length remains constant despite the movement of microtubules toward the spindle pole 



Any kinetochore that is attached to a microtubule undergoing such flux experiences a poleward force, which contributes to the generation of tension at the kinetochore in metaphase. Together with the kinetochore-based forces discussed above, flux also contributes to the poleward forces that move sister chromatids after they separate in anaphase.

The APC/C Triggers Sister-Chromatid Separation and the Completion of Mitosis

After M-Cdk has triggered the complex processes leading up to metaphase, the cell cycle reaches its climax with the separation of the sister chromatids at the metaphase-to-anaphase transition



Although M-Cdk activity sets the stage for this event, the anaphase-promoting complex (APC/C)  throws the switch that initiates sister-chromatid separation by ubiquitylating several mitotic regulatory proteins and thereby triggering their destruction  During metaphase, cohesins holding the sister chromatids together resist the poleward forces that pull the sister chromatids apart. Anaphase begins with the sudden loss of sister-chromatid cohesion, which allows the sisters to separate and move to opposite poles of the spindle. The APC/C initiates the process by targeting the inhibitory protein securin for destruction. Before anaphase, securin binds to and inhibits the activity of a protease called separase. The destruction of securin at the end of metaphase releases separase, which is then free to cleave one of the subunits of cohesin. The cohesins fall away, and the sister chromatids
separate. 





In addition to securin, the APC/C also targets the S- and M-cyclins for destruction, leading to the loss of most Cdk activity in anaphase. Cdk inactivation allows phosphatases to dephosphorylate the many Cdk target substrates in the cell, as required for the completion of mitosis and cytokinesis. If the APC/C triggers anaphase, what activates the APC/C? The answer is only partly known. As mentioned earlier, APC/C activation requires binding to the protein Cdc20. At least two processes regulate Cdc20 and its association with the APC/C. First, Cdc20 synthesis increases as the cell approaches mitosis, owing to an increase in the transcription of its gene. Second, phosphorylation of the APC/C helps Cdc20 bind to the APC/C, thereby helping to create an active complex. Among the kinases that phosphorylate and thus activate the APC/C is M-Cdk. Thus, M-Cdk not only triggers the early mitotic events leading up to metaphase, but it also sets the stage for progression into anaphase. The ability of M-Cdk to promote Cdc20–APC/C activity creates a negative feedback loop: M-Cdk sets in motion a regulatory process that leads to cyclin destruction and thus its own inactivation.

Unattached Chromosomes Block Sister-Chromatid Separation:
The Spindle Assembly Checkpoint

A spindle assembly checkpoint mechanism  ensures that cells do not enter anaphase until all chromosomes are correctly bi-oriented on the mitotic spindle. The spindle assembly checkpoint depends on a sensor mechanism that monitors the strength of microtubule attachment at the kinetochore, possibly by sensing tension. Any kinetochore that is not properly attached to the spindle sends out a diffusible negative signal that blocks Cdc20–APC/C activation throughout the cell
and thus blocks the metaphase-to-anaphase transition. When the last sister-chromatid pair is properly bi-oriented, this block is removed, allowing sister-chromatid
separation to occur.

The negative checkpoint signal depends on several proteins, including Mad2, which are recruited to unattached kinetochores . Detailed structural analyses of Mad2 suggest that the unattached kinetochore acts like an enzyme that catalyzes a change in the conformation of Mad2, so that Mad2, together with other proteins, can bind and inhibit Cdc20–APC/C. In mammalian somatic cells, the spindle assembly checkpoint determines the normal timing of anaphase. The destruction of securin in these cells begins moments after the last sister-chromatid pair becomes bi-oriented on the spindle, and anaphase begins about 20 minutes later. Experimental inhibition of the checkpoint mechanism causes premature sister-chromatid separation and anaphase. Surprisingly, the normal timing of anaphase does not depend on the spindle assembly checkpoint in some cells, such as yeasts and the cells of early frog and fly embryos. Other mechanisms, as yet unknown, must determine the timing of anaphase in these cells.

Chromosomes Segregate in Anaphase A and B


The sudden loss of sister-chromatid cohesion at the onset of anaphase leads to sister-chromatid separation, which allows the forces of the mitotic spindle to pull the sisters to opposite poles of the cell—called chromosome segregation. The chromosomes move by two independent and overlapping processes. The first, anaphase A, is the initial poleward movement of the chromosomes, which is accompanied by shortening of the kinetochore microtubules. The second, anaphase B, is the separation of the spindle poles themselves, which begins after the sister chromatids have separated and the daughter chromosomes have moved some distance apart.



Chromosome movement in anaphase A depends on a combination of the two major poleward forces described earlier. The first is the force generated by microtubule depolymerization at the kinetochore, which results in the loss of tubulin subunits at the plus end as the kinetochore moves toward the pole. The second is provided by microtubule flux, which is the poleward movement of the microtubules toward the spindle pole, where minus-end depolymerization occurs. The relative importance of these two forces during anaphase varies in different cell types: in embryonic cells, chromosome movement depends mainly on microtubule flux, for example, whereas movement in yeast  and vertebrate somatic cells results primarily from forces generated at the kinetochore. Spindle-pole separation during anaphase B depends on motor driven mechanisms similar to those that separate the two centrosomes in early mitosis. Plusend directed kinesin-5 motor proteins, which cross-link the overlapping plus ends of the interpolar microtubules, push the poles apart. In addition, dynein motors that anchor astral microtubule plus ends to the cell cortex pull the poles apart.

Although sister-chromatid separation initiates the chromosome movements of anaphase A, other mechanisms also ensure correct chromosome movements in anaphase A and spindle elongation in anaphase B. Most importantly, the completion of a normal anaphase depends on the dephosphorylation of Cdk substrates, which in most cells results from the APC/C-dependent destruction of cyclins. If M-cyclin destruction is prevented—by the production of a mutant form that is not recognized by the APC/C, for example—sister-chromatid separation generally occurs, but the chromosome movements and microtubule behavior of anaphase are abnormal. The relative contributions of anaphase A and anaphase B to chromosome segregation vary greatly, depending on the cell type. In mammalian cells, anaphase B begins shortly after anaphase A and stops when the spindle is about twice its metaphase length; in contrast, the spindles of  yeasts and certain protozoa primarily use anaphase B to separate the chromosomes at anaphase, and their spindles elongate to up to 15 times their metaphase length.


Segregated Chromosomes Are Packaged in Daughter Nuclei at Telophase

By the end of anaphase, the daughter chromosomes have segregated into two equal groups at opposite ends of the cell. In telophase, the final stage of mitosis, the two sets of chromosomes are packaged into a pair of daughter nuclei. The first major event of telophase is the disassembly of the mitotic spindle, followed by the re-formation of the nuclear envelope. Initially, nuclear membrane fragments associate with the surface of individual chromosomes. These membrane fragments fuse to partly enclose clusters of chromosomes and then coalesce to reform the complete nuclear envelope. Nuclear pore complexes are incorporated into the envelope, the nuclear lamina re-forms, and the envelope once again becomes continuous with the endoplasmic reticulum. Once the nuclear envelope has re-formed, the pore complexes pump in nuclear proteins, the nucleus expands, and the mitotic chromosomes are reorganized into their interphase state, allowing gene transcription to resume. A new nucleus has been created, and mitosis is complete. All that remains is for the cell to complete its division into two. We saw earlier that phosphorylation of various proteins by M-Cdk promotes spindle assembly, chromosome condensation, and nuclear-envelope breakdown in early mitosis. It is thus not surprising that the dephosphorylation of these same proteins is required for spindle disassembly and the re-formation of daughter nuclei in telophase. In principle, these dephosphorylations and the completion of mitosis could be triggered by the inactivation of Cdks, the activation of phosphatases, or both. Although Cdk inactivation—resulting primarily from cyclin destruction—is mainly responsible in most cells, some cells also rely on activation of phosphatases. In budding yeast, for example, the completion of mitosis depends on the activation of a phosphatase called Cdc14, which dephosphorylates
a subset of Cdk substrates involved in anaphase and telophase.

Summary


M-Cdk triggers the events of early mitosis, including chromosome condensation, assembly of the mitotic spindle, and bipolar attachment of the sister-chromatid pairs to microtubules of the spindle. Spindle formation in animal cells depends largely on the ability of mitotic chromosomes to stimulate local microtubule nucleation and stability, as well as on the ability of motor proteins to organize microtubules into a bipolar array. Many cells also use centrosomes to facilitate spindle assembly. Anaphase is triggered by the APC/C, which stimulates the destruction of the proteins that hold the sister chromatids together. APC/C also promotes cyclin destruction and thus the inactivation of M-Cdk. The resulting dephosphorylation of Cdk targets is required for the events that complete mitosis, including the disassembly of the spindle and the re-formation of the nuclear envelope.

The machinery of mitosis: Kinetechores, centrioles and chromosome pumps

https://vimeo.com/114072530

Mitosis-- Movie Narrative 1

Cell division is required for an organism to grow, mature, and maintain tissues. During the mitotic phase, a cell will undergo mitosis to form two new nuclei and then divide to form two new individual cells during cytokinesis.

Mitosis is the process of dividing the duplicated DNA of a cell into two new nuclei. Mitosis is split into distinct stages. The first stage is prophase; the DNA condenses, organizes, and the classic chromosome structure appears. Next comes prometaphase where microtubules attach to the chromosomes. This step is followed by metaphase where the chromosomes align. Metaphase is followed by anaphase where the chromosomes separate. Finally, during telophase nuclear membranes reappear around the two sets of chromosomes. Mitosis is now complete. After mitosis two new cells are formed by a process called cytokinesis.



Mitosis is only one part of what is called the cell cycle. For many eukaryotic cells, a cell is duplicated every 24 hours. Most of the life of a cell is spent in interphase. Interphase consists of three stages called G1, S, and G2.

G1 (or Gap 1) is the first growth stage of interphase. In G1, the cell grows to nearly its full size and performs many of its specific biochemical functions that aid the organism.

Next is the S (or synthesis) phase. This is an important stage, because it is during the S phase that DNA in the nucleus is replicated.

The cell next enters another growth stage called G2 (or Gap 2). It is during G2 that the cell finishes growing. Once the cell has duplicated DNA in the nucleus, and two centrosomes have appeared in the cytoplasm, mitosis can begin. For a typical eukaryotic cell this will last about 80 minutes.

During the first stage of mitosis, called prophase, we first see the classic chromosome structure. This occurs through a condensation process. At the same time, protein strands called microtubules appear from the centrosomes in animals. Finally, a structure found within the nucleus, the nucleolus, disappears.

Next, prometaphase begins when the nuclear membrane is broken down. At the same time, microtubule strands, or spindle fibers, are growing from the centrosomes. These strands attach to a protein structure called the kinetochore. One kinetochore is attached to the centromere of each sister chromatid.

Next comes metaphase. During this stage the sister chromatids align along the center of the cell so that both chromatids face toward opposite poles of the cell.

Now the sister chromatids are ready to be separated. This occurs during anaphase through a shortening of the microtubules attached to the kinetochores. Additionally, the poles of the cell move farther apart and cause increased separation of sister chromatids. At the end of anaphase, the sister chromatids have moved to the two ends of the cell.

Telophase is the final stage of mitosis. It is here the components of the new cells begin to appear. At this point the spindle fibers are broken up. A new nuclear membrane surrounds the chromosomes at the end of each cell. And the chromosomes uncoil and return to an uncondensed state. Mitosis is now complete. The formation of two cells is all that remains.

Following mitosis, the cell undergoes a process called cytokinesis. First the cell is compressed by a contractile ring that divides the cell in nearly equal halves. By now the organelles in the cell have been replicated, and are now divided between the two halves of the cell. This includes mitochondria, golgi bodies, and the rough ER. Plant cells also have chloroplasts. Once split, the two new cells are now fully in the G1 stage of interphase and ready again to begin their growth.

Let’s watch the process one more time. Mitosis begins with prophase. Notice the DNA condensing into chromosomes during this stage. Microtubules appear during prometaphase, and the nuclear membrane breaks down. Metaphase occurs when the chromosomes are aligned at the center of the cell. During anaphase the chromosomes are moving apart. The telophase stage is marked by the appearance of new nuclear membranes. This is the end of mitosis.

Finally, the splitting of the cell occurs during cytokinesis. The two new cells are now ready to grow and perform their specialized functions.



http://phys.org/news/2013-10-machinery-mitosis-kinetechores-centrioles-chromosome.html

(Phys.org) —At the cellular level, the mitotic spindle apparatus is arguably the most complicated piece of machinery in existence. Its basic function is to isolate and separate the chromosomes during cell division. A group of researchers at the University of North Carolina have been piecing together a model of the spindle and associated proteins which provides a way to visualize in detail exactly what might be going on. The group chose to simulate budding yeast cells because their entire spindle is comprised of only around 40 microtubules (MTs), compared to 100 times that amount in mammalian cells. Over the years the group has contributed to an emerging mechanical picture of the spindle wherein the MTs provide the compression elements, pericentric chromatin the elastic tension elements, and a proteinaceous kinetochore bridges the two polymers together. Their most recent paper, published in Current Biology, provides a new and detailed 3d map of the kinetochore region of the chromosome, and seeks to provides answers to the origins of the seemingly mysterious force that organizes the dividing cell.

Local force generation through the action of motor proteins, like kinesin and dynein, provides an important directional instructor to the spindle. However just as these components are likely not them main source of motility for large organelles like mitochondria, a more holistic view of spindle behavior suggests that a more diffuse, entropic influence constitutes the invisible hand that guides its overall dynamics. One potential source of force generation is provided by the chromosome pumps, if you will, that act like an osmotic contractile gel, expanding and constricting throughout mitosis—perhaps not completely unlike the recently revealed mechanism used in Herpes virus infection. Here the herpes DNA and associated matrix, under extreme pressure, literally blasts itself into the host cell.
The authors note that the entropic elasticity of chromosomal DNA can act to reel the spindle "arms" in to the spindle pole, just as one end of a spring recoils when the other end is pulled to a fxed point. This mechanism of entropic recoil has already been indicated to act in the segregation of replicated DNA in simpler bacteria. One can watch spindle behaviors under the microscope, and see these kinds of mechanisms at work, but they cannot be described very well in paper form by simply detailing lists of binding interactions. So these days, it now seems that in cell biology at least, the model provides the best way to move understanding forward, with parametrically-fed simulations providing the feedstock. Ideally, just like in parametric CAD, if the variables that specify the critical metrics are set up and linked properly, you can change one parameter, and those associated to it seamlessly follow suit without the need to create from scratch.
A Matlab/Simulink model was used to generate population measurements of spindle lengths for 16 left and 16 right kinetochore MTs, where each kinetochore has its own associated MT. The entire model contains spindle pole bodies (centrosomes), linked by the kinetochore MTs, with the main barrel structure comprised of coiled DNA tubes. These tubes are stitched together by cohesin rings and condensin linker molecules. In more recent models, the researchers are looking to constrain the unsolved structural details at the surface of the so-called inner kinetochore plate which resides at the kinetochore-MT interface.

In particular, they are looking to understand the distribution of critical molecules like Cse4 and Ndc80. To this end they created a model based on stochastic growth and shortening of spindles and kinetochore MTs. A cylindrical spindle with a diameter of 250nm was created based on geometry obtained form electron microscopy and tomography. Experimentally, flourophores were placed at the MT plus ends and used to simulate the distribution of Ndc80.
The results of these efforts are not best transmitted in word from. Nowadays collaborations like this are often headed up by artisans that recruit the biology and computer tech experts needed to create a visual simulation. The Harvard Biovisions series, or the illustrative DNA compaction video below are well known examples. The larger public may have to wait a little while to see the fruits of these new labors which seek to make the unseeable biology visible. The mitotic spindle may perhaps be the feature film that many haven't even realized they have been waiting for.


More information: A 3D Map of the Yeast Kinetochore Reveals the Presence of Core and Accessory Centromere-Specific Histone, Current Biology, 26 September 2013. dx.doi.org/10.1016/j.cub.2013.07.083
Abstract
The budding yeast kinetochore is ~68 nm in length with a diameter slightly larger than a 25 nm microtubule. The kinetochores from the 16 chromosomes are organized in a stereotypic cluster encircling central spindle microtubules. Quantitative analysis of the inner kinetochore cluster (Cse4, COMA) reveals structural features not apparent in singly attached kinetochores. The cluster of Cse4-containing kinetochores is physically larger perpendicular to the spindle axis relative to the cluster of Ndc80 molecules. If there was a single Cse4 (molecule or nucleosome) at the kinetochore attached to each microtubule plus end, the cluster of Cse4 would appear geometrically identical to Ndc80. Thus, the structure of the inner kinetochore at the surface of the chromosomes remains unsolved. We have used point fluorescence microscopy and statistical probability maps to deduce the two-dimensional mean position of representative components of the yeast kinetochore relative to the mitotic spindle in metaphase. Comparison of the experimental images to three-dimensional architectures from convolution of mathematical models reveals a pool of Cse4 radially displaced from Cse4 at the kinetochore and kinetochore microtubule plus ends. The pool of displaced Cse4 can be experimentally depleted in mRNA processing pat1Δ or xrn1Δ mutants. The peripheral Cse4 molecules do not template outer kinetochore components. This study suggests an inner kinetochore plate at the centromere-microtubule interface in budding yeast and yields information on the number of Ndc80 molecules at the microtubule attachment site.

1) http://vcell.ndsu.nodak.edu/animations/mitosis/movie-flash.htm


Origin of the cell nucleus, mitosis and sex: roles of intracellular coevolution

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2837639/

The transition from prokaryotes to eukaryotes was the most radical change in cell organisation since life began, with the largest ever burst of gene duplication and novelty. According to the coevolutionary theory of eukaryote origins, the fundamental innovations were the concerted origins of the endomembrane system and cytoskeleton, subsequently recruited to form the cell nucleus and coevolving mitotic apparatus, with numerous genetic eukaryotic novelties inevitable consequences of this compartmentation and novel DNA segregation mechanism. Physical and mutational mechanisms of origin of the nucleus are seldom considered beyond the long-standing assumption that it involved wrapping pre-existing endomembranes around chromatin. Discussions on the origin of sex typically overlook its association with protozoan entry into dormant walled cysts and the likely simultaneous coevolutionary, not sequential, origin of mitosis and meiosis.

The problem of nuclear origins therefore requires understanding coevolution of about 27 cell components and how they became functionally interlinked into the fundamentally novel eukaryotic life cycle

Peroxisomes, mitochondria, centrioles, cilia, and Golgi dictyosomes must also have originated prior to the last common ancestor of all extant eukaryotes.

This radical transformation of cell structure (eukaryogenesis) is the most complex and extensive case of quantum evolution in the history of life haha.

there are three crucial problems for understanding the origin of the nucleus [5]: (1) assembly of endomembranes around chromatin (the DNA-histone complex); (2) evolution of the nuclear pore complex (NPC), which crucially allows a channel between nucleoplasm and cytoplasm; and (3) origin of centromeres and mitotic spindle, without which nuclear chromosomes cannot be stably inherited.

origin of the cell nucleus cannot be understood in isolation from other major innovations of the eukaryotic cell; intracellular coevolution among different cell constituents that interact physically or that profoundly affect selective forces acting on each other is the key to understanding eukaryote origins

Intracellular coevolution of about a 100 novel properties is at the core of understanding eukaryogenesis.