Abiogenesis is impossible

http://web.pdx.edu/~niles/Lehman_Lab_at_PSU/Chem_460_560_files/CH460_Sp12_slides_final.pdf

There is a pdf of the book available on the internet with full color illustrations!

Yuri I Wolf and Eugene V Koonin (2007) On the origin of the translation system and the genetic code in the RNA world by means of natural selection, exaptation, and subfunctionalization, Biol Direct. 2007; 2: 14. Free access. Here the authors show that what I call ‘the Koonin threshold’ is based on the Eigen threshold. There is no mentioning of the 1,800 threshold, but there is a qualitative statement: “Indeed, we are unaware of translation being possible without the involvement of ribosomes, the complete sets of tRNA and aminoacyl-tRNA synthetases (aaRS), and (at least, for translation to occur at a reasonable rate and accuracy) several translation factors”. They also discuss ID, irreducible complexity.

Eugene V Koonin (2007) The cosmological model of eternal inflation and the transition from chance to biological evolution in the history of life, Biol Direct. 2007; 2: 15. (This is essentially Appendix B of the book.)

“The origin of life is one of the hardest problems in all of science, but it is also one of the most important. Origin-of-life research has evolved into a lively, interdisciplinary field, but other scientists often view it with skepticism and even derision. This attitude is understandable and, in a sense, perhaps justified, given the “dirty,” rarely mentioned secret: Despite many interesting results to its credit, when judged by the straightforward criterion of reaching (or even approaching) the ultimate goal, the origin of life field is a failure – we still do not have even a plausible coherent model, let alone a validated scenario, for the emergence of life on Earth.” (Koonin, p. 391). 
This text has been quoted by the uncommon descent intelligent design blog (Nov 13, 2011). The fact that the ID community is happy quoting Koonin without specifying a detailed ID alternative, demonstrates they are not interested in science, but only in attacking and ridiculing science. Why don’t IDists want to know how the designer did it?

All this is not to suggest that OORT [origins of replication and translation] is a problem of “irreducible complexity” and that the systems of replication and translation could not emerge by means of biological evolution. It remains possible that a compelling evolutionary scenario is eventually developed and, perhaps, validated experimentally. However, it is clear that OORT is not just the hardest problem in all of evolutionary biology but one that is qualitatively distinct from the rest. For all other problems, the basis of biological evolution, genome replication, is in place but, in the case of OORT, the emergence of this mechanism itself is the explanandum. Thus, it is of interest to consider radically different scenarios for OORT…
The MWO [“many worlds in one” – VJT] version of the cosmological model of eternal inflation could suggest a way out of this conundrum because, in an infinite multiverse with a finite number of distinct macroscopic histories (each repeated an infinite number of times), emergence of even highly complex systems by chance is not just possible but inevitable.

http://www.uncommondescent.com/intelligent-design/rna-the-origin-of-life-and-the-gullibility-of-the-science-media/#Note2

Abiogenesis is impossible

http://reasonandscience.heavenforum.org/t1279-abiogenesis-is-impossible

Evolution Impossible Dr. John F. Ashton, PhD page 41
For the first life to start from nonliving matter, thousands of specialized large complex molecules must somehow be synthesized in very large numbers from simple small inorganic molecules. These molecules then have to come together randomly over and over again until somehow the structure of the cell is formed. This remarkable and complex structure would still, however, not be alive. To become alive, hundreds of metabolic reactions would have to be initiated, with the metabolic intermediates already in place at just the right concentrations so that the reactions went the right way. Common sense tells us that these sorts of reactions just don’t happen by chance — in fact, we cannot even make them happen. To make the complex cell machine start up, we  have to change the concentration of hundreds of the metabolic intermediates back to just the right concentrations simultaneously. That is, we have to reinstate steady state nonequilibrium where the rate at which metabolites are formed is balanced perfectly with the rate they are required to be used by the next process. We know what to do, but even with our best technology we cannot achieve this — it is impossible. Once even a simple organism is dead it cannot be made alive again. This is a straightforward scientific observation. Evolution, however, requires not only the equivalent of a dead organism being made alive, but that the organism and its complex components and information systems must form in the first place by random processes. Then it must quickly be made alive before it has a chance to decompose or be damaged by other chemicals. Thus, the proponents of chemical evolution have to show that under the conditions that supposedly existed in a hypothetical primordial earth:

1. biomonomers (basic building block molecules) could form
2. biopolymers could form from these biomonomers
3. connected metabolic pathways could form
4. a live cell forms where chemical reactions are taking place in steady state ( i.e., perfectly balanced) nonequilibrium

To date, scientists have been able to replicate in the laboratory most of the reactions required for step 1. However, scientists have run into major problems trying to perform step 2. Small biopolymers only a fraction of the size required have been produced under ideal conditions using chemically reactive versions of nucleotides. These small, random molecules are a long, long way from the giant information encoded molecules required for life . The genetic information problem also has not been addressed in these experiments. Step 2 requires not only formation of biopolymers but also information to be encoded into these molecules to prepare for step 3. The evolutionary model requires this encoded information to occur as a result of nondirected random processes.

Dembski has shown mathematically that chance can be eliminated as a plausible explanation for a specified system when it exceeds the available probabilistic resources.  For the known universe, this is calculated
to be one chance in ten to the power 150, i.e., 10^150. The latter number is a 1 followed by 150 zeros. (Note 1 billion is 10^9, i.e., 1 followed by 9 zeros or 1,000,000,000.)

Consider the probability of a short, specifically coded protein molecule 100 amino acids in length arising by chance from its amino acid building blocks. To make the protein chain, all the amino acids must form a specific type of chemical bond known as a peptide bond with each other. However, other non-peptide bonds are possible and occur with approximately equal probability. This means that at any given site along the growing chain, the probability of having a peptide bond is one in two or ½. Therefore, the probability of having four peptide bonds in a four-link chain is ½ x ½ x ½ x ½ = (½)4 = 1/16 or 1 chance in 16. The probability of building a 100 amino acid chain with only peptide bonds is (½)99, which calculates to be around 1 chance in 10^30.

the chance of getting 100 L-amino acids forming a chain with only peptide bonds is now roughly one chance in 10^60 attempts

The probability of getting the right amino acid in the right site is 1 chance out of 20 possibilities. Therefore, the probability of forming a particular protein 100 amino acids long by chance would be (1/20)100, which is around 1 chance in 10^130.

A typical biological protein consists of about 300 amino acid units, and some are much longer. Biochemists at Cambridge University and MIT have published more detailed calculations of the probability of a functional sequence of amino acids arising by chance, and have come up with probabilities equivalent to finding a particular single atom in the universe!

From studies of single-celled organisms, scientists have estimated that the simplest possible living organism would require a genome containing a minimum of 250 to 400 genes. Thus, the improbability of life occurring in the simplest cells with the corresponding molecular complexity vastly exceeds 1 chance in 10^150. In other words, abiogenesis is absolutely impossible. That is, a living organism cannot arise by chance from nonliving matter.

plausible prebiotic synthesis

https://www.science.gov/topicpages/p/plausible+prebiotic+synthesis.html

What can we know about how life began ? 

http://reasonandscience.heavenforum.org/t1279p50-abiogenesis-is-impossible#5352

Nobody knows for sure. When it comes to historical sciences, nobody was there in the past to see what happened. But upon abductive reasoning, and the growing evidence and knowledge of chemistry, biochemistry, molecular biology, cell biology, evolutionary biology, genetics, epigenetics, and developmental biology, amount of knowledge about how life works, how it have might began and diversified,  is growing. That permits us more than ever before to make informed inferences. My take on abiogenesis is that we can make safe inferences based on what we DO  know.  Douglas Futuyma admits as much:

“Organisms either appeared on the earth fully developed or they did not. If they did not, they must have developed from preexisting species by some process of modification. If they did appear in a fully developed state, they must indeed have been created by some omnipotent intelligence” (Futuyma, 1983, p. 197).

In fact, Futuyma’s words underline a very important truth. He writes that when we look at life on Earth, if we see that life emerges all of a sudden, in its complete and perfect forms, then we have to admit that life was created, and is not a result of chance. As soon as naturalistic explanations are proven to be invalid, then creation is the only explanation left.

chemist Wilhelm Huck, professor at Radboud University Nijmegen
A working cell is more than the sum of its parts. "A functioning cell must be entirely correct at once, in all its complexity

To go from a bacterium to people is less of a step than to go from a mixture of amino acids to a bacterium. — Lynn Margulis. 

Mainstream scientific papers confirm indirectly that cells are irreducibly complex and interdependent. At the paper :

How Many Genes Can Make a Cell: The Minimal-Gene-Set Concept, the author writes : 
https://www.ncbi.nlm.nih.gov/books/NBK2227/
Several theoretical and experimental studies have endeavored to derive the minimal set of genes that are necessary and sufficient to sustain a functioning cell under ideal conditions, that is, in the presence of unlimited amounts of all essential nutrients and in the absence of any adverse factors, including competition. A comparison of the first two completed bacterial genomes, those of the parasites Haemophilus influenzae and Mycoplasma genitalium, produced a version of the minimal gene set consisting of ~250 genes. 

That means, a minimal number of genes, proteins, and metabolic network is essential to be there to give life a first go, as to turn the car's engine on. In the same manner, as if you are sitting in a car, and try to turn it on if the pistons in the car are missing, or even if a tiny electric cable is broken and you turn the car key, nothing goes. But life did not have a helping hand to fix the problem, check what part was missing, and pluck a broken cable in. For self-replication to start, a minimal set of proteins was absolutely essential to start self-replication:

http://reasonandscience.heavenforum.org/t1849-dna-replication-of-prokaryotes?highlight=dna+replication

So if only one protein, as helicase, for example, is missing, nothing goes. But why would a prebiotic soup produce a helicase protein by a lucky accident? Helicase by its own has no function, only when inserted and finely adjusted to do its job in the DNA replication mechanism.  Intelligent agents have foresight. Such agents can determine or select functional goals before they are physically instantiated. That's a huge problem for natural mechanisms, where no intelligence is in place. 

A minimal metabolic set was also required:

http://reasonandscience.heavenforum.org/t2371-how-cellular-enzymatic-and-metabolic-networks-point-to-design

a proeminent proposal, the so often mentioned RNA world, has also unbridgeable flaws, and cannot explain the origin of life adequately:

http://reasonandscience.heavenforum.org/t2024-the-rna-world-and-the-origins-of-life

The software/hardware in the cell, that is DNA, mRNA, RNA polymerase, tRNA's, the ribosome, tRNA Synthetases, protein chaperones etc, AND the software, that is the genetic code and translation mechanism,  had to emerge fully setup and TOGETHER, since one would have had no use without the other. That's a classic catch22 problem:

http://reasonandscience.heavenforum.org/t2221-the-hardware-and-software-of-the-cell-evidence-of-design?highlight=hardware

amongst many other catch22 situations that plague OOL researchers:

http://reasonandscience.heavenforum.org/t2059-catch22-chicken-and-egg-problems-in-biology-and-biochemistry

Furthermore, you need homeostasis and a functional signaling network right from the start:

http://reasonandscience.heavenforum.org/t2448-howintracellular-calcium-signaling-gradient-and-its-role-as-a-universal-intracellular-regulator-points-to-design?highlight=calcium

the ability of uptake of nutrients and its availability was also essential. That illustrates the tremendous difficulties that abiogenesis research faces. As for example: where did glucose come from?

http://reasonandscience.heavenforum.org/t2419-where-did-glucose-come-from-in-a-prebiotic-world?highlight=glucose

Then you need a set of proteins that use in their action centers metal clusters. To make them is an enormous feat and requires whole production lines and irreducible multistep biosynthesis processes: 

http://reasonandscience.heavenforum.org/t2445-amazing-molecular-assembly-lines-and-non-ribosomal-amino-acid-chain-formation-pathways-come-to-light

Another huge task is to create various cell codes, amongst them prominently the genetic code. The task is to create the code system itself, the director that plays the genetic piano, that is the gene expression network which determines which genes to turn on and off and express, find them in the genome, and express them at the right time, then encoding, transmission, and decoding of the information, and a translation system, where the genetic information is used to get useful proteins, the workhorses in the cell. The genetic code is more robust than one in a million:

 http://reasonandscience.heavenforum.org/t2363-the-genetic-code-insurmountable-problem-for-non-intelligent-origin

Furthermore, you need error check and repair systems all along the production line: DNA replication errors are reduced 10.000.000.000 times !! 

5ʹ => 3ʹ polymerization 1 in 100.000
3ʹ => 5ʹ exonucleolytic proofreading 1 in 100
Strand-directed mismatch repair 1 in 1000
Combined 1 in 10.000.000.000

Maintaining the genetic stability that an organism needs for its survival requires not only an extremely accurate mechanism for replicating DNA but also mechanisms for repairing the many accidental lesions that occur continually in DNA.

http://reasonandscience.heavenforum.org/t2043-dna-and-rna-error-checking-and-repair-amazing-evidence-of-design?highlight=error

the cell membrane could not have emerged as a simple vesicle, as Szostak et al try to popularize. Cell membranes are ENORMOUSLY COMPLEX, and membrane proteins for various functions are essential right from the start. Membranes and membrane proteins are interdependent and had to emerge together. I have various topics on the issue:

http://reasonandscience.heavenforum.org/f62-cell-membrane-and-membrane-proteins

Abiogenesis is a huge topic. There are essentially two possibilities. Either life was created, or it was not. If it was not created, all that is left, are random, unguided, lucky events that brought to the most complex self-replicating factory in the universe, full of molecular machines and production lines. 

Would you say that it is plausible that a tornado over a junkyard could produce a self-replicating machine, like John von Neumann's Universal Constructor?
Would you say that it is plausible that mindless random chance can write a book like a random letter generator using a computer pseudo-random number generator? if you see a message on a sand dune, like " John loves Sandy ". Would you intuitively and immediately recognize that someone past there a short time ago, and wrote the message on the sand dune? Or would you consider that rain and wind wrote the message randomly on the dune? The cell is far more complex than the most complex machine made by man, and the simplest cell stores as much information as contained in a CD. 

There are numerous other topics on the issue, which cannot be mentioned here. But this small resume gives a picture..... 

Sorry, I have not enough faith to be an atheist and believe, all this arose from a lucky accident. 

Abiogenesis is impossible

http://reasonandscience.heavenforum.org/t1279-abiogenesis-is-impossible

It’s Easy to Be an Atheist if You Ignore Science

Although the general public is disconcertingly unaware of it, it is a fact that scientists do not have even the slightest clue as to how life could have begun through an unguided naturalistic process absent the intervention of a conscious creative force.

Here are just a few well-chosen statements on the Origin of Life:

(2016) “[There is] collective cluelessness…those who say this is well worked out, they know nothing, nothing about chemical synthesis…Those who think that scientists understand the details of life’s origin are wholly uninformed. Nobody understands…when will the scientific community confess to the world that they are clueless on life’s origin, that the emperor has no clothes?” James Tour — Professor of Chemistry, Rice University (Synthetic chemist and among the top ten most cited chemists in the world)
(2011) “The Origin of Life field is a failure.” Eugene Koonin, microbiologist at the National Center for Biotechnology Information
(2011) “With respect to the Origin of Life, I find the more we learn about cells, the more complex they seem; they are just incredibly complex things, and to go from what we can see today and try to reason where it came from, I think is really impossible.” Lee Hartwell, Nobel Prize in Medicine, 2001
(2007) “How? [did life begin] I have no idea.” George Whitesides, Professor of Chemistry, Harvard University, Winner of the Priestley Medal in Chemistry (second only to the Nobel Prize)
(2001) “The origin of life appears to me as incomprehensible as ever, a matter for wonder but not for explication.” Franklin Harold, Professor Emeritus, Department of Biochemistry and Molecular Biology, Colorado State University
(1983) “In short, there is not a shred of objective evidence to support the hypothesis that life began in an organic soup here on earth.” Sir Fred Hoyle, distinguished British astronomer, physicist, mathematician (without question one of the greatest scientific minds of the 20th century)
(1981) “Since Science does not have the faintest idea how life on earth originated…it would only be honest to confess this to other scientists, to grantors, and to the public at large.” Hubert Yockey, physicist and renowned information theorist
Out of consideration for the reader, I won’t go back further than 35 years to illustrate the seamless ignorance of science and scientists regarding a naturalistic origin of life. Suffice it to say that not only has science not progressed in this area since Darwin published his famous treatise in 1859, but — on the contrary — it has slid backwards by many orders of magnitude.

SEPTEMBER 16, 2016 2:04 AM1
Were God Merely to ‘Exist,’ Our Prayers Would Be Meaningless
“God is a circle whose center is everywhere and circumference nowhere,” said Voltaire. Indeed, trying to describe God is like trying to...

What I mean by backwards becomes clear if we plot the Origin of Life dilemma on a standard x-y graph; with the horizontal X axis representing the understanding of a naturalistic origin of life from 1859 until the present. It is a straight line starting at zero (our understanding in 1859) and ending at zero (our understanding in 2016). Let the Y axis represent the level of understanding since 1859 of the magnitude of the problem that needs to be solved. In 1859 it was thought to be a relatively trivial issue (i.e. close to zero); however due to the astounding breakthroughs in genetics, biochemistry, and microbiology since then, the line of the Y axis is now off the graph.

As Biochemist Klaus Dose wrote: “Experimentation on the origin of life…has led to a better perception of the immensity of the problem of the origin of life on Earth rather than to its solution.” Researchers Carl Woese and Gunter Wachtershauser concur: “While we do not have a solution, we now have an inkling of the magnitude of the problem.”

Why are researchers having such difficulties discovering a naturalistic Origin of Life? Let’s let the aforementioned  and atheist microbiologist Eugene Koonin answer this question: “Certainly this is not due to a lack of experimental and theoretical effort, but to the extraordinary intrinsic difficulty and complexity of the problem. A succession of exceedingly unlikely steps is essential for the Origin of Life…these make the final outcome seem almost like a miracle.”

Translation for the lay-person: Discovering how unguided naturalistic forces could assemble a living cell — a molecular machine that is more sophisticated and functionally complex than anything human technology has ever produced — is a problem of nightmarish proportions.

Imagine a LEGO set designed to build a model of the Brooklyn Bridge, with hundreds of blocks specifically designed to construct it; imagine you are then assigned the task of finding a pathway to a successful assembly of the model using only unguided, naturalistic forces (i.e. heat, lightning, sunlight, wind, radiation, etc.) Would you agree with Koonin and describe that as a problem of “extraordinary intrinsic difficulty”? Actually, Koonin’s description is quite appropriate for the LEGO problem, but is a gross understatement when we are talking about something as frighteningly complex as a living cell and its DNA-based genetic code and digital information processing system:

The living cell is best thought of as a supercomputer – an information processing and replicating system of astonishing complexity. DNA is not a special life giving molecule but a genetic data bank that transmits its information using a mathematical code. Most of the workings of the cell are best described as…information, or software. Trying to make life by mixing chemicals in a test tube is like soldering switches and wires in an attempt to produce Windows 98. It won’t work because it addresses the problem at the wrong conceptual level. (Dr. Paul Davies, Origin of Life expert, Physicist, Arizona State University)

When one dispassionately contemplates the enormous difficulties involved in a naturalistic origin of life, it is not surprising at all that one often suggested solution is Intelligent Design or Divine Creation. In fact, any number of world class scientists themselves have brought up the issue:

“Abiogenesis [life from non-life] strikes many as virtually miraculous…you might get the impression from what I have written not only that the origin of life is virtually impossible, but that life itself is impossible…So what is the answer? Is life a miracle after all?” (Dr. Paul Davies)
“[We have no naturalistic explanation for] the origin of life, which is unknown so far…As long as the origin of life can’t be explained in natural terms, the hypothesis of an instant Divine creation of life cannot objectively be ruled out.” (Dr. Christian DeDuve, Nobel Prize-Medicine, 1974)
“There are only two possibilities as to how life arose. One is spontaneous generation arising to evolution; the other is a supernatural creative act of God. There is no third possibility.” (George Wald, Nobel Prize-Medicine, 1967)
“Although a biologist, I must confess I do not understand how life came about…I consider that life only starts at the level of a functional cell. The most primitive cells may require at least several hundred different specific biological macro-molecules. How such already quite complex structures may have come together remains a mystery to me. The possibility of the existence of a Creator, of God, represents to me a satisfactory solution to this problem.” (Dr. Werner Arber, Nobel Prize-Medicine, 1978)
“From my earliest training as a scientist I was very strongly brainwashed to believe that science cannot be consistent with any kind of deliberate creation. That notion has had to be very painfully shed. I am quite uncomfortable in the situation, the state of mind I now find myself in. But there is no logical way out of it; it is just not possible that life could have originated from a chemical accident.” (Chandra Wickramasinghe, mathematician, astronomer, astrobiologist – longtime collaborator of Sir Fred Hoyle)
“Indeed, such a theory [Intelligent Design] is so obvious that one wonders why it is not widely accepted as being self-evident. The reasons are psychological rather than scientific.” “A common sense interpretation of the facts suggests that a super intellect has monkeyed with the laws of physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature.” (Sir Fred Hoyle)
The Merriam-Webster Dictionary defines “atheism” as “a disbelief in the existence of the deity” or “the doctrine that there is no deity.” If one approaches the Origin of Life issue “objectively,” as Nobel Prize-winning biologist Christian DeDuve put it, there is no way that any rational person can rule out the very real possibility of a Creator of life. It is certainly reasonable to suggest or raise the possibility that the reason why scientists cannot find a naturalistic answer is because there is no naturalistic answer. Perhaps the reason why many people deny Intelligent Design as the answer to Origin of Life is a psychological reason not a scientific reason, as Sir Fred Hoyle has suggested. Perhaps the reason why a “common sense interpretation of the facts” suggests that a super-intellect has monkeyed with the universe, is because a super-intellect has monkeyed with the universe.

If a rational, truth-seeking individual is asked: “How did life begin; naturalistic, unguided forces or Divine Creation?” There are only two possible answers: (a) Divine Creation or (b) I don’t know, the jury is still out; but atheism – a denial of the existence of a Creator of life — is not possible anymore….unless, of course, as I stated in the title of this article, you are prepared to ignore science and scientists. And if so, you might just as well go and play children’s games and with children’s toys, like…..LEGO blocks.

https://www.algemeiner.com/2016/08/10/its-easy-to-be-an-atheist-if-you-ignore-science/

Bernd Rosslenbroich, On the Origin of Autonomy page 43

http://reasonandscience.heavenforum.org/t1279p50-abiogenesis-is-impossible#5666

The essential aspect of the theory presented here is that at some time and somehow life established biochemical functions that were not identical with the processes of the surrounding inorganic environment.

An essential part of early evolution must have been the origin of a system that carries and replicates information. Again, not much is known about the first principles of the biological storage of information. The essential characteristic of life is not so much the interacting matter but the information about how to use it to establish new islands of life. Information is immaterial, but it needs a material medium. Information is the source for building a higher degree of order than exists within the environment, as long as life functions are maintained. 

DNA repair systems contribute to the stabilization of the genome. They are present already from the prokaryotes on and eliminate mistakes in the genetic code with high efficacy. For example, the permanently working repair systems eliminate mistakes that result just from thermic fluctuations. Thus, every day about 5,000 purine bases from the DNA of each human cell are lost as their bondings to deoxyribose are hydrolyzed (depurination). In addition, spontaneous deaminations from cytosine to uracil occur through interactions with metabolic products and environmental influences (chemicals, light). The genetic autonomy must constantly be defended against destructive influences. In particular, the principle of the double helix enables repair because two copies of the information are available. One copy can be used as the backup copy to repair the other copy. Therefore, the principle of the double helix contributes to the independence and stability of the genome. 

If there was a prebiotic environment with some interaction of organic molecules as forerunners of the first cells, as some hypotheses assume, at some time a closed membrane must have appeared to form the principle of the cell. It must have enclosed a compartment for the concentration of biochemical molecules and their reactions. The encapsulation of such protoplasm in membranes is an essential question for any considerations about early evolution. If metabolic networks were somehow included in some sort of capsule, there would have been problems of permeation. Capsules or vesicles would have the effect of transferring the metabolic networks without a transition from an existence within a totally open surrounding into a completely sealed situation, which would bring these networks to a standstill. The question is how the remarkable balance between a relative separation from the environment and an intensive, but regulated, exchange of substances and energy evolved. emerged Modern cells solve this problem through a combination of the lipid bilayer with integrated membrane proteins. Not only is the transport through the membrane is regulated actively, partly through the consumption of energy, but also the properties of the lipid bilayer itself are regulated. Some authors postulate that from the beginning some sort of a protocell must have been involved.

I have noticed that both Dawkins and Coyne say that some sort of 'self-replicating molecule' is the most plausible - or perhaps least implausible - starting point. Do you happen to know what they mean by this? Is it a) RNA? b) something simpler, which then supposedly sets up a random mutation natural selection means of progression.

With regard to the latter I see Dawkins has made reference to Rebek's work in both River out of Eden and The Ancestor's Tale. (Wikipedia Julius_Rebek)

This doesn't seem to be of any use for getting to an RNA or RNA-DNA system.

Andrew

Andrew Chapman wrote:I have noticed that both Dawkins and Coyne say that some sort of 'self-replicating molecule' is the most plausible - or perhaps least implausible - starting point. Do you happen to know what they mean by this? Is it a) RNA? b) something simpler, which then supposedly sets up a random mutation natural selection means of progression.

With regard to the latter I see Dawkins has made reference to Rebek's work in both River out of Eden and The Ancestor's Tale. (Wikipedia Julius_Rebek)

This doesn't seem to be of any use for getting to an RNA or RNA-DNA system.

Andrew

Andrew, they mean the RNA hypothesis, which is not compelling at all. 

THE RNA WORLD,  AND THE ORIGINS OF LIFE

http://reasonandscience.heavenforum.org/t2024-the-rna-world-and-the-origins-of-life

Paul Davies The Algorithmic Origins of Life
Despite the conceptual elegance of the RNA world, the hypothesis faces problems, primarily due to the immense challenge of synthesizing RNA nucleotides under plausible prebiotic conditions and the susceptibility of RNA oligomers to degradation via hydrolysis 21 Due to the organizational structure of systems capable of processing algorithmic (instructional) information, it is not at all clear that a monomolecular system – where a single polymer plays the role of catalyst and informational carrier – is even logically consistent with the organization of information flow in living systems, because there is no possibility of separating information storage from information processing (that being such a distinctive feature of modern life). As such, digital–first systems (as currently posed) represent a rather trivial form of information processing that fails to capture the logical structure of life as we know it. 

Replicator first, and metabolism first scenarios
http://reasonandscience.heavenforum.org/t1428-replicator-first-and-metabolism-first-scenarios

No evidence that RNA molecules ever had the broad range of catalytic activities
http://reasonandscience.heavenforum.org/t2243-no-evidence-that-rna-molecules-ever-had-the-broad-range-of-catalytic-activities

The hardware and software of the cell, evidence of design
http://reasonandscience.heavenforum.org/t2221-the-hardware-and-software-of-the-cell-evidence-of-design

The origin of replication and translation and the RNA World
http://reasonandscience.heavenforum.org/t2234-the-origin-of-replication-and-translation-and-the-rna-world

Tom Robbins: The time argument is worthless. As over time, organic molecules break apart as fast as they form - thus the monkey's on a typewriter argument does not work, as the INFORMATION represented on the paper when they strike a key, disappears off the paper as they type. Given enough time, CERTAIN things will probably happen, but only things that are not impossible (and of course there was a finite amount of time from the creation of the earth). Nature can't create specified, dedicated, self-replicating, self-repairing, self-editing information - THE ONLY source that we know of that can do this, is MIND..

"We propose that the transition from non-life to life is unique and definable," added Davies. "We suggest that life may be characterized by its distinctive and active use of information, thus providing a roadmap to identify rigorous criteria for the emergence of life. This is in sharp contrast to a century of thought in which the transition to life has been cast as a problem of chemistry, with the goal of identifying a plausible reaction pathway from chemical mixtures to a living entity."

In a nutshell, the authors shift attention from the "hardware" – the chemical basis of life – to the "software" – its information content. To use a computer analogy, chemistry explains the material substance of the machine, but it won't function without a program and data. Davies and Walker suggest that the crucial distinction between non-life and life is the way that living organisms manage the information flowing through the system.
"When we describe biological processes we typically use informational narratives – cells send out signals, developmental programs are run, coded instructions are read, genomic data are transmitted between generations and so forth," Walker said. "So identifying life's origin in the way information is processed and managed can open up new avenues for research."

Paul Davies, an ASU Regents' Professor and director of the Beyond Center for Fundamental Concepts in Science, and Sara Walker, a NASA post-doctoral fellow at the Beyond Center.

What Davies missed to point out, is, that this information input could have come only from an intelligent designer.

http://www.dailygalaxy.com/my_weblog/2013/10/radical-theory-says-transition-from-inorganic-to-organic-life-was-based-on-information-not-chemistry.html

Admin wrote:"We propose that the transition from non-life to life is unique and definable," added Davies. "We suggest that life may be characterized by its distinctive and active use of information, thus providing a roadmap to identify rigorous criteria for the emergence of life. This is in sharp contrast to a century of thought in which the transition to life has been cast as a problem of chemistry, with the goal of identifying a plausible reaction pathway from chemical mixtures to a living entity."

In a nutshell, the authors shift attention from the "hardware" – the chemical basis of life – to the "software" – its information content. To use a computer analogy, chemistry explains the material substance of the machine, but it won't function without a program and data. Davies and Walker suggest that the crucial distinction between non-life and life is the way that living organisms manage the information flowing through the system.
"When we describe biological processes we typically use informational narratives – cells send out signals, developmental programs are run, coded instructions are read, genomic data are transmitted between generations and so forth," Walker said. "So identifying life's origin in the way information is processed and managed can open up new avenues for research."

Paul Davies, an ASU Regents' Professor and director of the Beyond Center for Fundamental Concepts in Science, and Sara Walker, a NASA post-doctoral fellow at the Beyond Center.

What Davies missed to point out, is, that this information input could have come only from an intelligent designer.

Hadn't this been pointed out by 1966 at the Wistar Institute, and certainly by Hubert Yockey by 1981?

Andrew Chapman wrote:

Hadn't this been pointed out by 1966 at the Wistar Institute, and certainly by Hubert Yockey by 1981?

Yes.

A calculation of the probability of spontaneous biogenesis by information theory
Hubert P. Yockey
The Darwin-Oparin-Haldane “warm little pond” scenario for biogenesis is examined by using information theory to calculate the probability that an informational biomolecule of reasonable biochemical specificity, long enough to provide a genome for the “protobiont”, could have appeared in 109 years in the primitive soup. Certain old untenable ideas have served only to confuse the solution of the problem. Negentropy is not a concept because entropy cannot be negative. The role that negentropy has played in previous discussions is replaced by “complexity” as defined in information theory. A satisfactory scenario for spontaneous biogenesis requires the generation of “complexity” not “order”. Previous calculations based on simple combinatorial analysis over estimate the number of sequences by a factor of 105. The number of cytochrome c sequences is about 3·8 × 10^61. The probability of selecting one such sequence at random is about 2·1 ×10^65. The primitive milieu will contain a racemic mixture of the biological amino acids and also many analogues and non-biological amino acids. Taking into account only the effect of the racemic mixture the longest genome which could be expected with 95 % confidence in 109 years corresponds to only 49 amino acid residues. This is much too short to code a living system so evolution to higher forms could not get started. Geological evidence for the “warm little pond” is missing. It is concluded that belief in currently accepted scenarios of spontaneous biogenesis is based on faith, contrary to conventional wisdom.
http://www.sciencedirect.com/science/article/pii/0022519377900443

By chance? - Not a chance !!

http://reasonandscience.heavenforum.org/t1279p50-abiogenesis-is-impossible#5764

Single proteins do not have any function on their own unless interconnected correctly in a living cell. In order for life to begin naturally, all essential proteins required for life to start would have had to emerge randomly on a prebiotic earth, protein super-complexes like ribosomes would have had to join the subparts together to get the right protein-protein interactions, like lock and key. A miracle would have had to prevent them to be burned by UV radiation. Then start to interconnect in the correct order to create a functional metabolic network and multi-protein production lines , where the joint venture of several enzymes began to produce functional products, hand them over to carrier mechanisms, tag them in order to be transported to the right locations. Somehow, all this would have had to begin in a protected environment, so a protective envelope would have had to exist.

Somehow, that envelope had to create a homeostatic environment, diminishing the calcium concentration in the cell 10000 times below the external environment, to permit signaling. At the same time, a signaling code would have had to be established, and immediately begin to function, with a common agreement between sender and receiver................energy supply would have been a major problem, since almost all life forms depend on the supply of glucose, which is a product of complex metabolic pathways, and not readily available on a prebiotic earth. Most proteins require active metal clusters in their reaction centers.

These clusters are in most cases ultracomplex, each cluster had to have the right atoms interconnected in the right way, and get the correct 3-dimensional form. They require the complex uptake of the basic materials, like iron and sulfur, molybdenum, and complex biosynthesis processes, and after the correct assembling, the insertion in the right way and form inside the proteins. All these processes require energy, in form of ATP, not readily available - since ATP is the product of complex nano-factories, like ATP synthase - which by themselves depend on a proton gradient. Sorry------- not by chance !!

From Primordial Soup to the Prebiotic Beach

An interview with exobiology pioneer, Dr. Stanley L. Miller, University of California San Diego

1n 1953, a University of Chicago graduate student named Stanley Miller working in Harold Urey's lab flipped a switch sending electric current through a chamber containing a combination of methane, ammonia, hydrogen and water. The experiment yielded organic compounds including amino acids, the building blocks of life, and catapulted a field of study known as exobiology into the headlines. Since that time a new understanding of the workings of RNA and DNA, have increased the scope of the subject. Moreover, the discovery of prebiotic conditions on other planets and the announcement of a bacterial fossil originating on Mars has brought new attention to the study of life's origins. I spoke with Dr. Miller in his lab at UCSD about the field he has helped to make famous, exobiology.

Let start with the basics. Can you give a simple definition of exobiology?
The term exobiology was coined by Nobel Prize-winning scientist Joshua Lederberg. What it means is the study of life beyond the Earth. But since there's no known life beyond the Earth people say its a subject with no subject matter. It refers to the search for life elsewhere, Mars, the satellites of Jupiter and in other solar systems. It is also used to describe studies of the origin of life on Earth, that is, the study of pre-biotic Earth and what chemical reactions might have taken place as the setting for life's origin.

Some 4.6 billion years ago the planet was a lifeless rock, a billion years later it was teeming with early forms of life. Where is the dividing line between pre-biotic and biotic Earth and how is this determined?
We start with several factors. One, the Earth is fairly reliably dated to 4.55 billion years. The earliest evidence for life was 3.5 billion years based on findings at the Apex formation in Western Australia. A new discovery reported in the journal Nature indicates evidence for life some 300 million years before that. We presume there was life earlier, but there is no evidence beyond that point.
We really don't know what the Earth was like three or four billion years ago. So there are all sorts of theories and speculations. The major uncertainty concerns what the atmosphere was like. This is major area of dispute. In early 1950's, Harold Urey suggested that the Earth had a reducing atmosphere, since all of the outer planets in our solar system- Jupiter, Saturn, Uranus and Neptune- have this kind of atmosphere. A reducing atmosphere contains methane, ammonia, hydrogen and water. The Earth is clearly special in this respect, in that it contains an oxygen atmosphere which is clearly of biological origin.
Although there is a dispute over the composition of the primitive atmosphere, we've shown that either you have a reducing atmosphere or you are not going to have the organic compounds required for life. If you don't make them on Earth, you have to bring them in on comets, meteorites or dust. Certainly some material did come from these sources. In my opinion the amount from these sources would have been too small to effectively contribute to the origin of life.

So while these are potential sources of organic compounds they are not essential for the creation of life on Earth?
As long as you have those basic chemicals and a reducing atmosphere, you have everything you need. People often say maybe some of the special compounds came in from space, but they never say which ones. If you can make these chemicals in the conditions of cosmic dust or a meteorite, I presume you could also make them on the Earth. I think the idea that you need some special unnamed compound from space is hard to support.
You have to consider separately the contributions of meteors, dust and comets. The amount of useful compounds you are going to get from meteorites is very small. The dust and comets may provide a little more. Comets contain a lot of hydrogen cyanide, a compound central to prebiotic synthesis of amino acids as well as purines. Some HCN came into the atmosphere from comets. Whether it survived impact, and how much, are open to discussion. I'm skeptical that you are going to get more than a few percent of organic compounds from comets and dust. It ultimately doesn't make much difference where it comes from. I happen to think prebiotic synthesis happened on the Earth, but I admit I could be wrong.
There is another part of the story. In 1969 a carbonaceous meteorite fell in Murchison Australia. It turned out the meteorite had high concentrations of amino acids, about 100 ppm, and they were the same kind of amino acids you get in prebiotic experiments like mine. This discovery made it plausible that similar processes could have happened on primitive Earth, on an asteroid, or for that matter, anywhere else the proper conditions exist.



Doesn't the Panspermia theory looks at the question of ultimate origins of life in a slightly different way?
That's a different controversy. There are different versions of the theory. One idea is that there was no origin of life, that life, like the universe, has always existed and got to the Earth through space. That idea doesn't seem very reasonable since we know that the universe has not always existed, so life has to happen some time after the big bang 10 or 20 billion years ago.
It may be that life came to Earth from another planet. That may or may not be true, but still doesn't answer the question of where life started. You only transfer the problem to the other solar system. Proponents say conditions may have been more favorable on the other planet, but if so, they should tell us what those conditions were.
Along these lines, there is a consensus that life would have had a hard time making it here from another solar system, because of the destructive effects of cosmic rays over long periods of time.

What about submarine vents as a source of prebiotic compounds?
I have a very simple response to that . Submarine vents don't make organic compounds, they decompose them. Indeed, these vents are one of the limiting factors on what organic compounds you are going to have in the primitive oceans. At the present time, the entire ocean goes through those vents in 10 million years. So all of the organic compounds get zapped every ten million years. That places a constraint on how much organic material you can get. Furthermore, it gives you a time scale for the origin of life. If all the polymers and other goodies that you make get destroyed, it means life has to start early and rapidly. If you look at the process in detail, it seems that long periods of time are detrimental, rather than helpful.

Can you review with us some of the history and basic background of your original prebiotic experiments?
In the 1820's a German chemist named Woeller announced the synthesis of urea from ammonium cyanate, creating a compound that occurs in biology. That experiment is so famous because it is considered the first example where inorganic compounds reacted to make a biological compound. They used to make a distinction between organic, meaning of biological origin, and inorganic- CO₂, CO and graphite. We now know that there is no such distinction.
However, it remained a mystery how you could make organic compounds under geological conditions and have them organized into a living organism. There were all sorts of theories and speculation. It was once thought that if you took organic material, rags, rotting meat, etc, and let it sit, that maggots, rats etc. would arise spontaneously. It's not as crazy as it seems, considering DNA hadn't been discovered. It was then reasonable to hold those views if you consider living organisms as protoplasm, a life substance. This all changed in 1860 when Pasteur showed that you don't get living organisms except from other living organisms. This disproved the idea of spontaneous generation.
But spontaneous generation means two things. One is the idea that life can emerge from a pile of rags. The other is that life was generated once, hundreds of millions of years ago. Pasteur never proved it didn't happen once, he only showed that it doesn't happen all the time.
A number of people tried prebiotic experiments. But they used CO₂F, nitrogen and water. When you use those chemicals, nothing happens. It's only when you use a reducing atmosphere that things start to happen.

Who came up with the idea of the reducing atmosphere?
Oparin, a Russian scientist, began the modern idea of the origin of life when he published a pamphlet in 1924. His idea was called the heterotrophic hypothesis: that the first organisms were heterotrophic, meaning they got their organic material from the environment, rather than having to make it, like blue-green algae. This was an important idea. Oparin also suggested that the less biosynthesis there is, the easier it is to form a living organism. Then he proposed the idea of the reducing atmosphere where you might make organic compounds.
He also proposed that the first organisms were coacervates, a special type of colloid. Nobody takes that last part very seriously anymore, but in 1936, this was reasonable since DNA was not known to be the genetic material..
In 1951, unaware of Oparin's work, Harold Urey came to the same conclusion about the reducing atmosphere. He knew enough chemistry and biology to figure that you might get the building blocks of life under these conditions.

Tell us about the famous electrical discharge experiment.
The experiments were done in Urey's lab when I was a graduate student. Urey gave a lecture in October of 1951 when I first arrived at Chicago and suggested that someone do these experiments. So I went to him and said, "I'd like to do those experiments". The first thing he tried to do was talk me out of it. Then he realized I was determined. He said the problem was that it was really a very risky experiment and probably wouldn't work, and he was responsible that I get a degree in three years or so. So we agreed to give it six months or a year. If it worked out fine, if not, on to something else. As it turned out I got some results in a matter of weeks. 

Next he exposed the gases to a continuous electrical discharge ("lightning"), causing the gases to interact. Water-soluble products of those reactions then passed through a condenser and dissolved in the mock ocean. The experiment yielded many amino acids and enabled Miller to explain how they had formed. For instance, glycine appeared after reactions in the atmosphere produced simple compounds - formaldehyde and hydrogen cyanide. Years after this experiment, a meteorite that struck near Murchison, Australia, was shown to contain a number of the same amino acids that Miller identified and in roughly the same relative amounts. Such coincidences lent credence to the idea that Miller's protocol approximated the chemistry of the prebiotic earth. More recent findings have cast some doubt on that conclusion.

---

You must have been excited to get such dramatic results so quickly, and with what, at the time, must have seemed like an outlandish hypothesis?
Oh yes. Most people thought I was a least a little bit crazy. But if you look at methane/ammonia vs CO₂/nitrogen there was no doubt in my mind. It was very clear that if you want to make organic compounds it would be easier with methane. It's easy to say that but it is quite a bit more difficult to get organized and do the experiment.
The surprise of the experiment was the very large yield of amino acids. We would have been happy if we got traces of amino acids, but we got around 4 percent. Incidentally, this is probably the biggest yield of any similar prebiotic experiment conducted since then. The reason for that has to do with the fact that amino acids are made from even simpler organic compounds such as hydrogen cyanide and aldehydes.
That was the start. It all held together and the chemistry turned out to be not that outlandish after all.

What was the original reaction to your work in the science community?
There was certainly surprise. One of the reviewers simply didn't believe it and delayed the review process of the paper prior to publication. He later apologized to me. It was sufficiently unusual, that even with Urey's backing it was difficult to get it published. If I'd submitted it to "Science" on my own, it would still be on the bottom of the pile. But the work is so easy to reproduce that it wasn't long before the experiment was validated.
Another scientist was sure that there was some bacterial contamination of the discharge apparatus. When you see the organic compounds dripping off the electrodes, there is really little room for doubt. But we filled the tank with gas, sealed it, put it in an autoclave for 18 hours at 15 psi. Usually you would use 15 minutes. Of course the results were the same.
Nobody questioned the chemistry of the original experiment, although many have questioned what the conditions were on pre-biotic Earth. The chemistry was very solid.

How much of a role did serendipity play in the original setup?
Fortunately, Urey was so adamant at the time about methane that I didn't explore alternate gas mixtures. Now we know that any old reducing gases will do. CO₂/hydrogen and nitrogen will do the trick, although not as well.
There was some serendipity in how we handled the water. If we hadn't boiled it and run it for a week, we wouldn't have gotten such good yields of amino acids. We knew right away that something happened rather quickly because you could see a color change after a couple of days.
The fact that the experiment is so simple that a high school student can almost reproduce it is not a negative at all. That fact that it works and is so simple is what is so great about it. If you have to use very special conditions with a very complicated apparatus there is a question of whether it can be a geological process.

The original study raised many questions. What about the even balance of L and D (left and right oriented) amino acids seen in your experiment, unlike the preponderance of L seen in nature? How have you dealt with that question?
All of these pre-biotic experiments yield a racemic mixture, that is, equal amounts of D and L forms of the compounds. Indeed, if you're results are not racemic, you immediately suspect contamination. The question is how did one form get selected. In my opinion, the selection comes close to or slightly after the origin of life. There is no way in my opinion that you are going to sort out the D and L amino acids in separate pools. My opinion or working hypothesis is that the first replicated molecule had effectively no asymmetric carbon

You are talking about some kind of pre-RNA?
Exactly a kind of pre-RNA. RNA has four asymmetric carbons in it. This pre-RNA must have somehow developed into RNA. There is a considerable amount of research now to try and figure out what that pre-RNA compound was, that is, what was the precursor to the RNA ribose-phosphate.

Peter E. Nielsen of the University of Copenhagen has proposed a polymer called peptide nucleic acid (PNA) as a precursor of RNA. Is this is where PNA comes in?
Exactly, PNA looks prebiotic. Currently that is the best alternative to ribose phosphate. Whether it was the original material or not is another issue.

Can you clarify one thing? Have all of the amino acids been synthesized in pre-biotic experiments, along with all the necessary components for making life?
Just turning on the spark in a basic pre-biotic experiment will yield 11 out of 20 amino acids. If you count asparagine and glutamine you get thirteen basic amino acids. We don't know how many amino acids there were to start with. Asparagine and glutamine, for example, do not look prebiotic because they hydrolyze. The purines and pyrimidines can alos be made, as can all of the sugars, although they are unstable.

Your original work was published only a month apart from Watson and Crick's description of the DNA molecule. How has the field of molecular biology influenced the field of exobiology?
The thing that has probably changed the outlook the most is the discovery of ribozymes, the catalytic RNA. This means you can have an organism with RNA carrying out both the genetic functions and catalytic functions. That gets around the problem of protein synthesis, which is this incredibly complicated thing. There is a problem with RNA as a prebiotic molecule because the ribose is unstable. This leads us to the pre-RNA world.
The idea of the pre-RNA world is essentially the same as the RNA world, except you have a different molecule that replicates. Another thing worth remembering is that all these pre-biotic experiments produce amino acids. To have these amino acids around and not use them in the first living organism would be odd. So the role of amino acids in the origin of life is unknown but still likely.

Tell us about your recent work and the lagoon idea.
The primitive Earth had big oceans, but it also had lakes, lagoons and beaches. Our hypothesis is that the conditions may have been ideal on these beaches or drying lagoons for prebiotic reactions to occur, for the simple reason that the chemicals were more concentrated in these sites than in the middle of the ocean.

Is this because of the temperatures and also the presence of minerals as well?
Temperature is an important factor. Minerals have been thought by some to play a role in the origin of life, but they really haven't done much for us so far. People talk about how minerals might have helped catalyze reactions, but there are few examples where the mineral makes any difference.
Our most recent research tackled the problem of making pyrimidines- uracil and cytosine, in prebiotic conditions. For some reason it just doesn't work very well under dilute conditions. We showed that it works like a charm once you get things concentrated and dry it out a bit. This changed my outlook on where to start looking for prebiotic reactions.
Another example is our work with co-enzyme A. The business end of co-enzyme A is called pantetheine. We showed you could make this under these kind of pre-biotic "dry beach" conditions. We found that you didn't need it to be very hot, you can make it at 40 degrees C. This indicates the ease with which some of this chemistry can take place.

Temperature seems to be a talking point regarding prebiotic hypotheses.
We know we can't have a very high temperature, because the organic materials would simply decompose. For example, ribose degrades in 73 minutes at high temperatures, so it doesn't seem likely. Then people talk about temperature gradients in the submarine vent. I don't know what these gradients are supposed to do. My thinking is that a temperature between 0 and 10 degrees C would be feasible. The minute you get above 25 degrees C there are problems of stability.

How does the discovery of the Martian meteorite factor in to the discussion? Are you convinced these are the fossilized remains of extraterrestrial microorganisms?
I think the data is interesting and suggestive, but not yet conclusive. Let's accept that the meteorite does come from Mars. You have apparently got very small bacterial fossils also iron sulfide and magnetite sitting next to each other. Then there are these PAHs (polycyclic aromatic hydrocarbons). All of this is suggestive but not compelling.
There are just two possibilities. Either there was life on Mars or there was not. I have no problem with the idea of life on Mars, the question remains whether this evidence is adequate. If it is correct, it has an implication for one of the big questions of prebiotic research. That is, is it easy or difficult to produce life from prebiotic compounds in prebiotic conditions? It seems that it would be difficult on Mars. If it turns out to be the case on Mars, where the conditions do not look very favorable, then it should apply to anywhere in the universe, or any planet with a suitable atmosphere and temperature.

Can you tell us about the field of exobiology today in context of the world of science research?
It is a very small field. There is a society, the International Society for the Study of the Origin of Life. It has only 300 members, a rather small society. My own lab is part of program called NSCORT (NASA Specialized Center of Research and Training). This program is conducted in close cooperation with NASA and supports five researchers along with graduate students, post-docs and undergraduate students.
The more important research are the experiments these days, rather than the trading of ideas. Good ideas are those that when reduced to an experiment end up working. Our approach is to do experiments and demonstrate things, not just talk about possibilities.

What advice do you have for students interested in pursuing studies in exobiology?
Well we are talking about solving chemical problems. Therefore a background in basic chemistry is essential along with knowledge in the fields of organic chemistry, biochemistry and some background in geology and physics. Exobiology is a small field with a lot of interaction. It is one of few fields where an undergraduate would be able to work with top people in the field almost immediately.
This interview was conducted in October, 1996


1. https://web.archive.org/web/20080518054852/http://www.accessexcellence.org/WN/NM/miller.php

Cell morphology cannot be read out of genomic sequences alone.

http://reasonandscience.catsboard.com/t1279p50-abiogenesis-is-impossible#5954

Molecules into Cells: Specifying Spatial Architecture 1
2005 Dec
Spatial organization is perhaps the most conspicuous quality of cells and organisms, and also the most elusive. The dissection of life into its molecular constituents—the genes, enzymes, and lipid bilayers—necessarily starts with the destruction of spatial order. Yet we know that most complex functions, such as motility and division, depend on having the right molecules in their proper places. Architecture is what ultimately distinguishes a living cell from a soup of the chemicals of which it is composed. How cells generate, maintain, and reproduce their spatial organization is central to any understanding of the living state. 1 Organization, like other aspects of physiology and function, manifests the instructions encoded in the genes. Genes, acting individually or through elaborate regulatory networks, specify the structure of living matter and ensure its persistence; ultimately it is the genes that build cells.

If a protein deleted or altered by mutation plays a role in morphogenesis, the mutant's form or organization may well be affected. Examples from both prokaryotes and eukaryotes run into the hundreds . Such mutants are immensely valuable in dissecting morphogenetic pathways, but they do not show that cell form and organization are explicitly spelled out in the genetic instructions.

My comment: This paper was published in 2005. We know today that epigenetic mechanisms play a major role, if not a central role, in conjunction with genes, in how Cells are intracellularly organized.

Is cellular architecture explicitly spelled out by genes, and if so, how? If not, how is spatial organization passed from one generation to the next? How do molecules find the correct location in the cell space? What is the origin of large-scale order, as illustrated by the mitotic spindle or the endomembrane system of eukaryotic cells? How do multitudes of molecules reproducibly come together into cellular forms, which in turn serve as the targets of natural selection?

Targeting sequences direct proteins to the plasma membrane, nucleus, mitochondria, or lysosomes. Certain proteins and mRNAs are transported individually to particular locations in cell space, and this localization depends on having an appropriate sequence. Transport vesicles recognize specific target membranes, such as the Golgi, vacuole, or plasma membrane, with the aid of SNARE proteins. But there is much more to growth and division than manufacturing the parts. A rod-shaped cell must also elongate with constant diameter, construct an efficient apparatus to partition its chromosomes, locate its midpoint, lay down a septum, and undergo fission. In eukaryotic cells, targeted vesicle fusion requires, in addition to the SNAREs, both a delivery system and a secretory apparatus. This is all elaborate choreography. Genes specify the molecular parts, not their arrangement into a higher order.

Question: How did this targeting sequence emerge? And not only the sequence by itself but also the mechanisms of recognition of these target sequences. There has to be an information source, an encoder, a communication channel, a decoder (or receiver), and a user. The information source generates the information to be transmitted; the encoder transforms the information into a suitable message form for transmission over the communication channel; and the decoder performs the inverse operation of the encoder, or approximately so, for the user at the other end of the channel.

As Perry Marshall explains :

1. A code
2. An encoder that obeys the rules of a code
3. A message that obeys the rules of the code
4. A decoder that obeys the rules of the code

The rules of any communication system are always defined in advance by a process of deliberate choices. There must be prearranged agreement between sender and receiver, otherwise communication is impossible. By definition, a communication system cannot evolve from something simpler because evolution itself requires communication to exist first. You can’t make copies of a message without the message, and you can’t create a message without first having a language. And before that, you need intent. A code is an abstract, immaterial, nonphysical set of rules. There is no physical law that says ink on a piece of paper formed in the shape T-R-E-E should correspond to that large leafy organism in your front yard. You cannot derive the local rules of a code from the laws of physics, because hard physical laws necessarily exclude choice. On the other hand, the coder decides whether “1” means “on” or “off.” She decides whether “0” means “off” or “on.” Codes, by definition, are freely chosen. The rules of the code come before all else. These rules of any language are chosen with a goal in mind: communication, which is always driven by intent. That being said, conscious beings can evolve a simple code into a more complex code—if they can communicate in the first place. But even simple grunts and hand motions between two humans who share no language still require communication to occur. Pointing to a table and making a sound that means “table” still requires someone to recognize what your pointing finger means.

The paper continues:
For the purposes of cell biology, let me define self-organization as the emergence of supramolecular order from the interactions among numerous molecules that obey only local rules, without reference to an external template or global plan.

My comment: Does a rule not demand a ruler ?

The structure of the self-assembled complex is wholly specified by the structures of its parts and is therefore implicit in the genes that specify those parts: natural selection crafted those genes to specify parts that assemble into a functional complex.

My comment: Did you perceive the AD-HOC explanation? Natural selection? How does the author know this? He doesn't. Furthermore, there was no natural selection prior life began.


"Although the general public is disconcertingly unaware of it, it is a fact that scientists do not have even the slightest clue as to how life could have begun through an unguided naturalistic process absent the intervention of a conscious creative force.
Here are just a few well-chosen statements on the Origin of Life:
- (2016) “[There is] collective cluelessness…those who say this is well worked out, they know nothing, nothing about chemical synthesis…Those who think that scientists understand the details of life’s origin are wholly uninformed. Nobody understands…when will the scientific community confess to the world that they are clueless on life’s origin, that the emperor has no clothes?” (James Tour — Professor of Chemistry, Rice University (Synthetic chemist and among the top ten most cited chemists in the world))
- (2011) “The Origin of Life field is a failure.” (Eugene Koonin, microbiologist at the National Center for Biotechnology Information)
- (2011) “With respect to the Origin of Life, I find the more we learn about cells, the more complex they seem; they are just incredibly complex things, and to go from what we can see today and try to reason where it came from, I think is really impossible.” (Lee Hartwell, Nobel Prize in Medicine, 2001)
- (2007) “How? [did life begin] I have no idea.” (George Whitesides, Professor of Chemistry, Harvard University, Winner of the Priestley Medal in Chemistry (second only to the Nobel Prize))
- (2001) “The origin of life appears to me as incomprehensible as ever, a matter for wonder but not for explication.” (Franklin Harold, Professor Emeritus, Department of Biochemistry and Molecular Biology, Colorado State University)
- (1983) “In short, there is not a shred of objective evidence to support the hypothesis that life began in an organic soup here on earth.” (Sir Fred Hoyle, distinguished British astronomer, physicist, mathematician (without question one of the greatest scientific minds of the 20th century))
- (1981) “Since Science does not have the faintest idea how life on earth originated…it would only be honest to confess this to other scientists, to grantors, and to the public at large.” (Hubert Yockey, physicist and renowned information theorist)
As Biochemist Klaus Dose wrote: “Experimentation on the origin of life…has led to a better perception of the immensity of the problem of the origin of life on Earth rather than to its solution.” Researchers Carl Woese and Gunter Wachtershauser concur: “While we do not have a solution, we now have an inkling of the magnitude of the problem.”
Why are researchers having such difficulties discovering a naturalistic Origin of Life? Let’s let the aforementioned and atheist microbiologist Eugene Koonin answer this question: “Certainly this is not due to a lack of experimental and theoretical effort, but to the extraordinary intrinsic difficulty and complexity of the problem. A succession of exceedingly unlikely steps is essential for the Origin of Life…these make the final outcome seem almost like a miracle.”
Translation for the lay-person: Discovering how unguided naturalistic forces could assemble a living cell — a molecular machine that is more sophisticated and functionally complex than anything human technology has ever produced — is a problem of nightmarish proportions.
When one dispassionately contemplates the enormous difficulties involved in a naturalistic origin of life, it is not surprising at all that one often suggested solution is Intelligent Design or Divine Creation. In fact, any number of world class scientists themselves have brought up the issue:
“Abiogenesis [life from non-life] strikes many as virtually miraculous…you might get the impression from what I have written not only that the origin of life is virtually impossible, but that life itself is impossible…So what is the answer? Is life a miracle after all?” (Dr. Paul Davies)
“[We have no naturalistic explanation for] the origin of life, which is unknown so far…As long as the origin of life can’t be explained in natural terms, the hypothesis of an instant Divine creation of life cannot objectively be ruled out.” (Dr. Christian DeDuve, Nobel Prize-Medicine, 1974)
“There are only two possibilities as to how life arose. One is spontaneous generation arising to evolution; the other is a supernatural creative act of God. There is no third possibility.” (George Wald, Nobel Prize-Medicine, 1967)
“Although a biologist, I must confess I do not understand how life came about…I consider that life only starts at the level of a functional cell. The most primitive cells may require at least several hundred different specific biological macro-molecules. How such already quite complex structures may have come together remains a mystery to me. The possibility of the existence of a Creator, of God, represents to me a satisfactory solution to this problem.” (Dr. Werner Arber, Nobel Prize-Medicine, 1978)
“From my earliest training as a scientist I was very strongly brainwashed to believe that science cannot be consistent with any kind of deliberate creation. That notion has had to be very painfully shed. I am quite uncomfortable in the situation, the state of mind I now find myself in. But there is no logical way out of it; it is just not possible that life could have originated from a chemical accident.” (Chandra Wickramasinghe, mathematician, astronomer, astrobiologist – longtime collaborator of Sir Fred Hoyle)
“Indeed, such a theory [Intelligent Design] is so obvious that one wonders why it is not widely accepted as being self-evident. The reasons are psychological rather than scientific.” “A common sense interpretation of the facts suggests that a super intellect has monkeyed with the laws of physics, as well as with chemistry and biology, and that there are no blind forces worth speaking about in nature.” (Sir Fred Hoyle)
If a rational, truth-seeking individual is asked: “How did life begin; naturalistic, unguided forces or Divine Creation?” There are only two possible answers: (a) Divine Creation or (b) I don’t know, the jury is still out; but atheism – a denial of the existence of a Creator of life — is not possible anymore….unless, of course, as I stated in the title of this article, you are prepared to ignore science and scientists. And if so, you might just as well go and play children’s games and with children’s toys, like…..LEGO blocks."

1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1306800/

Understanding the processes of life’s origin is mind-bogglingly complex. To date, we have not even dented the metaphoric surface of the mysteries of the origin of life; for example, we are still grappling with the question of which came first, metabolism, genetics or vesicles? How else could the details of life’s origins be unearthed?


1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4500147/

Artificial intelligence and humanoids, compared to the human body

DESIGN PRINCIPLES of a human mimetic humanoid
The fundamental concept underlying our design is to consider the human mechanism, which contrasts with the conventional engineering approach used in the design of existing humanoids. For at least the last two millennia, human beings have endeavored to understand the systems and mechanisms that make up the human body, such as the principles of muscle control, the sensory nervous system that connects the brain and the body, the mechanisms of learning in the brain, and the accomplishment of the simple act of walking.
http://robotics.sciencemag.org/content/2/13/eaaq0899

Kenshiro is the most advanced Human humanoid MADE IN JAPAN. mimetic humanoid DESIGN CRITERIA were on the basis of the mechanisms in the human body. Its musculoskeletal systems are as close as possible to that of a human.  The researchers in Japan have been refining robotic parts to mimic human parts and assembling them to make whole robots that move as closely as possible to the ways humans move.
https://techxplore.com/news/2017-12-team-japan-advanced-humanoid-robot.html

“These robots are inherently cross-disciplinary, involving advanced locomotion and manipulation, biomechanics, artificial intelligence, machine vision, perception, learning and cognitive development, as well as behavioral studies.
https://www.telegraph.co.uk/science/2017/12/20/human-like-robots-yet-able-play-badminton-perform-sit-ups-blessed/

2017 PAL Robotics launches TALOS, a fully electrical humanoid robot with joint torque sensors and EtherCAT communication technology that can manipulate up to 6Kg payload in each of its grippers.
https://en.wikipedia.org/wiki/Humanoid_robot

SMARTLY DESIGNED Carefully crafted
http://blog.pal-robotics.com/talos-robot-the-new-humanoid-from-pal-robotics/

It took humanity two thousand years, and the best minds to conceptualize and develop human like robots, which are despite all the intelligence employed to create them, far from close to the complexity and perfection of the human body.

The human body:
When we arrive on this earth we are endowed with the most perfect, the most efficient, and the best constructed machine ever devised – our body. A machine beautifully engineered and constructed with the best materials with no planned obsolescence. Constructed with material of superb quality destined with proper use to last long periods of time.

The body's computer, the brain, is by far the most sophisticated, the finest constructed, the most efficient computer that has ever been or ever will be designed. No man-made computer can approach the efficiency of the computer each of us has.

- 37,2 trillion Cells
- each Cell hosts 2,3 billion molecular machines ( proteins )
- The  human brain: 86 billion neurons
- The bit capacity of the human brain:  10^8,342 bits , exceeds the bit capacity of the entire universe at 10^120 bits
- the storage capacity of one human brain is equivalent to 10^8,419 modern computers
- the human brain’s memory capacity:  1,000 terabytes (for comparison, the 19 million volumes in the US Library of Congress represents about 10 terabytes of data)

http://reasonandscience.catsboard.com/t2697-topics-on-the-structural-complexity-of-the-human-body?highlight=human

No intelligence required to make it ?

Organic vs non-organic

If you see blueprint with precise instructions how to build a factory, including all machines, production lines, energy generator, computers etc.:  Would you intuitively and immediately recognize that someone very intelligent elaborated the blueprint upon knowledge, information, intuition, inventive power, experience, and reason?  Or would you consider that ink randomly was thrown on a piece of paper, and somehow, the ink formed letters, lines, graphics, instructions, tables, etc. ? Even the most basic biological cell maintaining the most basic functions of life requires far more complex information to make the simplest Cell than man-made machines.  Would you say that it is plausible that random, unguided, natural events have enough statistical probability to create and give rise to the most sophisticated self-replicating factory in the universe? -  

Michael Denton writes in Evolution: A Theory In Crisis:
“To grasp the reality of life as it has been revealed by molecular biology, we must magnify a cell a thousand million times until it is twenty kilometres in diameter and resembles a giant airship large enough to cover a great city like London or New York. What we would then see would be an object of unparalleled complexity and adaptive design. On the surface of the cell, we would see millions of openings, like the port holes of a vast space ship, opening and closing to allow a continual stream of materials to flow in and out. If we were to enter one of these openings we would find ourselves in a world of supreme technology and bewildering complexity. The complexity of the simplest known type of cell is so great that it is impossible to accept that such an object could have been thrown together suddenly by some kind of freakish, vastly improbable, event. Such an occurrence would be indistinguishable from a miracle. The cell is a veritable micro-miniaturized factory containing thousands of exquisitely designed pieces of intricate molecular machinery, made up altogether of one hundred thousand million atoms, far more complicated than any machine built by man and absolutely without parallel in the non-living world. 

The cell contains an informational code system and programming languages like our alphabet or a computer code, more versatile than C, Visual Basic, or PHP, and more robust and error-free than any other code system out of 1 million alternatives? -  using a communication protocol which wastes far less space than TCP/IP and is more robust than Ethernet? -  using furthermore a collection of rules and regularities of information coding for instructional complex texts? - defined by alphabet, grammar, a collection of punctuation marks and regulatory sites, and semantics?  and then uses that  code system  to create a blueprint for a self-replicating factory, which requires about 1500 books, each with 300 pages, 300 thousand characters per book, each containing the precise complex instructions and information to create this factory,  and stored in the smallest storage device possible and known, a trillion times denser than a CD? How is it, that you would recognize immediately, that a simple message on a sand dune required intelligence, but above description of the simplest imaginable biological cell does not require a designer ?!

Objection: Comparing living cells to man-made self-replicating machines,  and books is a false analogy
Answer:  Talking about life getting together is similar to talking about cars forming themselves, or even basic computer programs making themselves. These things are not just improbable, they are impossible without intelligence. 
Marcello Barbieri writes in his book: Code Biology A New Science of Life, page 28
Molecular biology has proved that there is a genetic code in every cell and that genes and proteins are molecular artifacts because they are manufactured by molecular machines. Coding and artifact-making, in other words, take place both in our society and inside the cell, and this does create a parallel between culture and molecular biology. 
In other words. Intelligence produces self-replicating machines and books. And so only intelligence can produce life, that depends on coded information, proteins, and molecular machines. 

If the analogy of two phenomena are very close and striking while at the same time, the cause of ONE of the phenomenon is very obvious; it becomes scarcely possible to refuse to admit the action of an analogous cause of the other phenomenon, though (the cause of the other phenomenon is) not so obvious in itself"
--- in "Preliminary Discourse on the Study of Natural Philosophy", London, Longman, Rees, Orme, Brown and Green, 1831, page 149.

When you see that:
the way genetic information encoded in the DNA is exactly the same as what we humans would do to elaborate a blueprint to build a factory, full of complex machines, compartments, production lines, computers, etc;
the way that the nucleus communicates with its ribosome is similar to how we humans have designed computers to communicate with one another,
then one has to AT LEAST stop and wonder whether some intelligent being has designed the genetic code and made the communication system between the nucleus and its ribosomes.... 

Perry Marshall writes in the book Evolution 2.0:
Although this is not a conclusive proof of the existence of God, it should AT LEAST make one STOP and THINK about the possibility of the existence of God....

COMPILATION OF QUOTES ON THE COMPLEXITY OF A CELL AND THE SCIENTIFIC MYSTERY OF LIFE'S ORIGIN
By Ashby L. Camp

http://theoutlet.us/Quotesoncomplexityofcellandoriginoflife.pdf

Carl Sagan, astronomer, "Life," in 10 Encyclopaedia Britannica: Macropaedia, 15th ed. (Chicago: Encyclopaedia Britannica, 1974), 893-894:

A living cell is a marvel of detailed and complex architecture. Seen through a microscope there is an appearance of almost frenetic activity. On a deeper level it is known that molecules are being synthesized at an enormous rate. . . . The information content of a simple cell has been estimated as around 1012 bits, comparable to about a hundred million pages of the Encyclopaedia Britannica.

Thanks to experiments with snake meat, Francesco Redi realized 350 years ago: All life comes from an egg!

Francesco "Baltazar"Redi (18 February 1626 – 1 March 1697) was an Italian physician, naturalist, biologist and poet. He is referred to as the "founder of experimental biology" 1

Are there any animals that spontaneously develop from mud or sand? The idea of such "generation" was in circulation since Aristotle until Francesco Redi refuted it 350 years ago. Redi's famous experiment in 1668, was one of the first steps in refuting "spontaneous generation" - a theory also known as Aristotelian Abiogenesis.

Since Aristotle, it has been said that in addition to sexual and vegetative reproduction, there is a third type of reproduction. Spontaneous generation. This refers to the idea that so-called "lower" animals such as insects, worms, shells, crabs, amphibians - the naturalist Réaumur counted in his "Mémoires pour servir à l 'histoire des insectes" (1734-1742) even the crocodile to - arising from mud, sand or decaying cadaver ("generatio ex putrefactione"). In a letter, Redi then reported to his friend Carlo Dati about his investigations of the phenomenon and told him the result that even "lower" animals, unlike all other creatures, were created by sexual procreation. Period. And now the battle cry was: "Omnia ex ovo!" I mean, life comes out of an egg.

Francesco Redi was a member of the Accademia del Cimento. This union had written Galileo Galilei's motto "provando e riprovando" on the flag, which says: prove and prove by further experiments again. And so the researcher diversified his experiment several times to confirm the validity of his discovery by means of control and counter-experiments.

Redi's discovery spread quickly. However, the followers of the doctrine of the original generation, which was always repeated by the Fathers of the Church, were not so easily defeated. And indeed, only four years after Redi's experiments, the Dutchman Antoni van Leeuwenhoek discovered the infusoria, microscopic animals, in his water droplets from eaves and puddles using his light microscope. With these, the traditionalist defenders of spontaneous generation rejoiced, it would be an impossibility to observe eggs. The arguments went on for a long time, until in 1861 Louis Pasteur succeeded, through experiments with gooseneck-shaped test tubes, to prove definitively that the time-honoured idea of ​spontaneous generation had become obsolete.

This happened 200 years after Redis sensational discovery. But even the ingenious Pasteur speculated in 1878 in an unpublished note about whether the spontaneous emergence of life should not be somehow possible. After all, she could have played a role at the beginning of life on our planet. No reason from today's point of view to ridicule the supposedly authoritarian hearing traditionalists who persist despite Redis discovery on the traditional "knowledge" of a primordial generation. Or is it clearer today how organic life could have evolved four billion years ago from the primordial soup of inorganic matter? It is therefore only logical that abiogenesis ("A-Bio-Genesis"), ie spontaneous generation, has remained an important research topic in chemistry of our time.

By the late 1870s, most scientists agreed that all organisms arose from the reproduction of preexisting organisms, and the concept of spontaneous generation had become history. 2

1. https://en.wikipedia.org/wiki/Francesco_Redi
2. Biology A Self-Teaching Guide Second Edition, page 19

Abiogenesis is a mount insurmountable - by all means.

Biological Cells are a self-replicating factory with the ability of self-replication of the entire factory once ready, to respond to changing environmental demands and regulate its metabolic pathways, regulate and coordinate all cellular processes, such as molecule and building block biosynthesis according to the cells demands, depending on growth, and other factors. The ability of uptake of nutrients, to be structured, internally compartmentalized and organized, being able to check replication errors and minimize them, and react to stimuli, and changing environments. That's is, the ability to adapt to the environment is a must right from the beginning. If just ONE single protein or enzyme - of many - is missing, no life. The study below lists functional annotation descriptions ( proteins, enzymes). If just ONE of these 561 is missing, no life. If topoisomerase II or helicase are missing - no replication - no perpetuation of life. Let us suppose a fortunate accident would sort out, amongst over 500 known different types of amino acids, the 20 types, used for life. Not only would it have to select the right ones amongst these over 500 amino acids which can exist and come in two configurations -  left, and right-handed chiral directions. They would have to be joined somehow to the same assembly site on early earth in sufficient quantities. The availability of these amino acids would have to be synchronized so that at some point, either individually or in combination, they are all available at the same time. The selected parts must all be made available at the same ‘construction site,’ perhaps not simultaneously but certainly, at the time, they are needed.

Once there, each protein with an average of about 300 amino acids would have to be produced, each with its individual specific function. At least 561 of the right, life essential proteins. Each complementary to the others, in right shape and size, able to interact with others like lock and key. The individual proteins had to be mutually compatible, that is, ‘well-matched’ and capable of properly ‘interacting’: even if subsystems or parts are put together in the right order, they also need to interface correctly. If they are not joined together in a functional manner, no deal. Nothing will go. The parts must be coordinated in just the right way: even if all of the parts of a system are available at the right time, it is clear that the majority of ways of assembling them will be non-functional or irrelevant.

As a comparison: Imagine a print machine. If it had 561 parts, each had to be produced individually and then assembled together. If the cartridge is not extant, the printer cannot work. Same with biological Cells. Another major hurdle is, if there is no energy source, the printer won't work. Same with biological Cells. While we can plug an energy cable into a socket and connect the printer to an energy source, there was non-available on a prebiotic earth. Cells use extremely complex metabolic pathways to produce their energy in form of ATP.

So, somehow, early earth produced these 561 complementary proteins.  And if there is no energy in form of ATP molecules to drive each protein through phosphorylation, nothing goes. So besides these proteins, ATP would have had to be around, ready to do its job. The problem is: It takes ATP to make proteins. But it takes proteins to make ATP. What came first? Another major problem would be - these polypeptides would be exposed to UV radiation,      ( there was no protective UV shield yet on the atmosphere ), so rather than stabilize, they would disintegrate quickly.  All this would have had to happen by self-assembly, spontaneously by orderly aggregation and sequentially correct manner without external direction.

In Cells, fermentation is a very slow process to make ATP. In bacteria and eukaryotes, the production of ATP is done through the central metabolism pathways, but they yield only a small quantity of ATP. The major producers are ATP synthase, veritable nano turbines, another marvel, one of the most amazing molecular machines. And if they are not coupled to a proton gradient, which requires other complex proteins which create it, no deal. And if there are no mechanisms to produce the molecules used as burning fuel like glucose to drive the process of proton gradient formation, no deal either.  

Cells use complex molecular machines and EXTREMELY complex multistep pathways to synthesize each of these 20 amino acids, and enzymes measure and sensor with feedback loops precisely the rate of amino acids that are required of each AA type like a computer, and produces the right quantity, accordingly. And in order to economize energy, it recycles used proteins. Proteasomes are protein complexes which degrade unneeded or damaged proteins by proteolysis, a chemical reaction that breaks peptide bonds, and so, amino-acids are recycled, which is an enormous economy of energy expenditure to synthesize new proteins. Amino acids come from keto acids and other compounds in the Reductive Tricarboxylic Acid (rTCA) Cycle, a central metabolic pathway in all life forms. The problem here again is another catch22. It takes nine complex proteins of the reverse Citric Acid Cycle to fix carbon and make it available for the production of the carbon backbone of amino acids. But it takes this process to make the proteins and enzymes required in the Reductive Tricarboxylic Acid (rTCA) Cycle. We have only considered the make of amino acids, not taking in consideration, that to make the other basic building blocks of life, namely RNA, DNA, hydrocarbons and fatty acids, each constitutes in other catch22 situations.

So the problem of the arrangement of the right amino acid sequence to make just one protein is the least of all problems that origin of life research faces, they are so many, that we can say with confidence: Abiogenesis is a no-no.

A minimal estimate for the gene content of the last universal common ancestor
19 December 2005
A truly minimal estimate of the gene content of the last universal common ancestor, obtained by three different tree construction methods and the inclusion or not of eukaryotes (in total, there are 669 ortholog families distributed in 561 functional annotation descriptions ( proteins, enzymes) , including 52 which remain uncharacterized) This set of 669/561 sequence/function categories can be considered as the truly minimal estimate for the gene content of LUCA. LUCA  does not appear dramatically different from extant life

Minimal  gene content of the first biological cell = 561 functional annotation descriptions = that means, it cannot be reduced further = irreducibly complex

- Replication/recombination/repair/modification
- Transcription/regulation
- transslation/ribosome
- RNA processing
- cell division
- thermoprotection
- signaling
- proteolysis
- Transport/membrane
- Electron transport
- Metabolism

The gene content of the last universal common ancestor (LUCA) with respect to DNA processing (replication, recombination, modification and repair) contains a wide range of functions. The following families/functions are identified:

- DNA polymerase
- excinuclease ABC
- DNA gyrase
- topoisomerase
- NADdependent DNA ligase
- DNA helicases
- mismatch repair MutS  and MutT
- endonucleases, RecA
- chromosome segregation SMC
- methyltransferase, methyladenine

I have not enough faith to believe in non-guided mechanisms to explain the origin of life. Do you?

Catch22 Origin of Life problems - checkmake for Abiogenesis

Lynn Margulis:
To go from a bacterium to people is less of a step than to go from a mixture of amino acids to a bacterium.

This is very true. But we can go a step further:

To go from the existence of the basic building blocks on early earth to Cell assembly of complex carbohydrates, lipids, proteins, nucleic acids, metals and seven non-metal elements from monomers is less of a step than to go from the existence of these small organic molecules synthesized in Cells to a fully working self-replicating Cell. This might be surprising, but not when someone knows the efforts taken by cells to synthesize these building blocks, which is truly remarkable.

One of the most dramatic evidence why abiogenesis fails is the fact that to make these basic building blocks of life, the cell machinery which is made upon these very basic building blocks must be fully set up and operating. That creates a catch22 situation:

It takes Proteins to make the basic building blocks of life. But it takes the basic building blocks of life to make proteins.
It takes ATP to make proteins. But it takes proteins to make ATP.
It takes proteins to make amino acids. But it takes amino acids to make proteins
It takes DNA to make proteins. But it takes proteins to make DNA.
Cell duplication and DNA replication is essential for the survival and perpetuation of all living things. It takes over 30 specialized irreducible proteins for DNA replication. But it takes the DNA to RNA transcription and RNA translation to make proteins. What came first?
It takes proteins to make the error-check and correction proteins that reduce the DNA replication error rate 10.000.000.000 times. These error check and correction proteins had to be checked too. How did the process start?
But it takes the DNA transcription and translation to make proteins. What came first?
It takes a fully setup signalling network for cells to adapt to ecological variations, like heat-shock proteins to adapt to climate variation and temperatures. How did that system start, if it is non-functional, if not fully setup?
It takes fully synthesized Fe/S ( Iron sulphur) clusters for a majority of proteins used in oxidation-reduction reactions, essential for all life forms. But it takes complex uptake and synthesis processes to make these metal clusters through veritable nano-molecular non-ribosomal peptide synthetase (NRPS) assembly lines for iron uptake. What came first: These manufacturing assembly lines, or the proteins that make them?
The central metabolic pathways like glycolysis or the Citric Acid cycle are essential to make Adenine triphosphate ( ATP ),  the energy currency in the cell, and amino acids, the basic building blocks of proteins. These metabolic pathways use enzymes, which are made through ATP and amino acids. How did these pathways emerge?
It takes DNA and proteins to make phospholipids for Cell membranes. But the Cell membrane must be fully setup and permit a closed, protected environment, for the very own processes to operate that synthesize Cell membranes.
The Lipid membrane would be useless without membrane proteins but how could membrane proteins have emerged in the absence of functional membranes?

Carbohydrates are metabolized to provide energy and are stored in muscle and liver as glycogen. Six-carbon glucose molecules are degraded by a series of chemical reactions to three-carbon pyruvate by the reactions of glycolysis; pyruvate.  The core structure of the metabolic network is very similar across all organisms. Centrally located within this network are the sugar-phosphate reactions of glycolysis and the pentose phosphate pathway. Together with the overlapping reactions of the Entner–Doudoroff pathway and of the Calvin cycle, they provide the precursor metabolites required for the synthesis of RNA, DNA, lipids, energy and redox coenzymes and amino acids—key molecules required for life.

Cells use hierarchical levels of organization, where the function and proper set up of the higher level depend on the lower level. And that lower level, as shown above, depends on irreducible biochemical synthesis processes. That is an all or nothing business, which could not be setup if not by intelligent setup.